【摘要】 目的:观察iNKT细胞对哮喘小鼠肺树突状细胞（LDCs）表面分子和促炎性细胞因子表达水平的影响。方法:24只野生型BALB/c小鼠随机分为正常对照组、哮喘组和过继转移组,每组8只; 12只CD1d^-/--BALB/c小鼠随机分为CD1d^-/-对照组和CD1d^-/-哮喘组,每组6只。哮喘组、过继转移组和CD1d^-/-哮喘组小鼠以卵清白蛋白致敏和激发,正常对照组和CD1d^-/-对照组以等量PBS替代,其中过继转移组在第1次激发前1 h给予iNKT细胞尾静脉注射。采用流式细胞仪检测各组小鼠LDCs及表面分子MHCⅡ、CD80、CD86和CD40的表达水平; ELISA法检测LDCs体外培养上清液IL-12p70、IL-6、TNF-α和IL-10水平。结果:过继转移组小鼠LDCs数量和MHCⅡ、CD40、CD80、CD86表达水平及LDCs体外培养上清液IL-12p70、IL-6、TNF-α水平明显高于哮喘组（P<0. 05或P<0. 01）; CD1d^-/-哮喘组小鼠LDCs数量和MHCⅡ、CD40、CD80、CD86表达水平及LDCs体外培养上清液IL-12p70、IL-6、TNF-α水平明显低于哮喘组（P<0. 05或P<0. 01）,但明显高于正常对照组和CD1d^-/-对照组（P<0. 05或P<0. 01）。结论:iNKT细胞可以增强哮喘小鼠LDCs表面分子和促炎性细胞因子的表达水平。更多还原

【Abstract】 Objective: To investigate the effect of iNKT cells on the expression of surface molecules and proinflammatory cytokines of lung dentritic cells（ LDCs） in an OVA-induced mouse model of asthma. Methods: Twenty-four wild type（ WT） BALB/c mice were randomly divided into normal control group（ n = 8）,asthma group（ n = 8） and adoptive transfer group（ n = 8）. Twelve CD1d^-/--BALB/c mice were randomly divided into CD1d^-/-control group（ n = 6） and CD1d^-/-asthma group（ n = 6）. The mouse model of asthma was induced through sensitization with intraperitoneal administration of ovalbumin（ OVA） and intranasal challenge in asthma group,adoptive transfer group,and CD1d^-/-asthma group,whereas in normal control group and CD1d^-/-control group where PBS was used instead. Adoptive transfer of iNKT cells in adoptive transfer group and PBS in asthma group were carried out 1 h prior the first challenge. The number of LDCs and expression of MHC Ⅱ,CD40,CD80,CD86 on LDCs were detected by flow cytometry. Levels of IL-12 p70,IL-6,TNF-α and IL-10 in the culture supernatants of LDCs were measured by ELISA. Results: The number of LDCs and expression of MHCⅡ,CD40,CD80,CD86 on LDCs,as well as the levels of IL-12 p70,IL-6 and TNF-α in culture supernatants of LDCs from adoptive transfer group were markedly increased,compared with asthma group（ P < 0. 05 or P < 0. 01）. Moreover,the number of LDCs,MHCⅡ⁺LDCs,CD40⁺LDCs,CD80⁺LDCs,CD86⁺LDCs as well as the levels of IL-12 p70,IL-6,TNF-α in culture supernatants of LDCs from CD1d^-/-asthma group were significantly decreased compared with asthma group（ P<0. 05 or P<0. 01）,but was markedly increased compared with control group and CD1d^-/-control group（ P<0. 05 or P<0. 01）. Conclusion: iNKT cells can enhance the expression of surface molecules and proinflammatory cytokines of LDCs in an OVA-induced mouse model of asthma.更多还原