【摘要】 目的观察蒙药乳腺-Ⅰ(M-Ⅰ)号对乳腺增生症大鼠乳腺组织中与抗氧化损伤相关的蛋白过氧化物酶(Prdx)-1,谷胱甘肽S转移酶(GSTP),超氧化物歧化酶(SOD)表达的影响。方法雌性未孕Wistar大鼠,随机选出8只作为正常对照组,其余动物连续25 d每天肌肉注射苯甲酸雌二醇(0. 5 mg/kg) 1次,随后连续5 d每天肌肉注射黄体酮(4 mg/kg) 1次,复制大鼠乳腺增生模型;同时,正常对照组大鼠肌肉注射橄榄油。模型复制成功后,随机分为:模型对照组,给予生理盐水灌胃; M-Ⅰ号低剂量组和高剂量组别给予M-Ⅰ号0. 5、3. 0 g/kg灌胃。给药结束后,观察大鼠乳腺组织病理学特征,检测大鼠乳腺组织中Prdx-1,GSTP,GS,SOD含量。结果与正常对照组大鼠比较,模型对照组乳腺组织Prdx-1及GSTP表达水平均显著升高(P<0. 01或P<0. 05),SOD表达显著降低(P<0. 05),乳腺组织增生改变明显。与模型对照组大鼠比较,M-Ⅰ号各剂量组大鼠乳腺组织Prdx-1表达明显减少,GSTP、SOD的表达明显升高(P<0. 01或P<0. 05);乳腺组织增生改变较模型对照组明显减轻。结论 M-Ⅰ号对乳腺增生大鼠有较好的治疗作用,其治疗机制涉及对Prdx-1、GSTP和SOD蛋白的调节作用。更多还原

【Abstract】 Objective To observe the effects of Mongolian Remedy Ru Xian-Ⅰ (M-Ⅰ) on anti-oxidation-related proteins per-oxiredoxin (Prdx)-1,glutathione s-transferase P (GSTP) and superoxide Dismutase (SOD) in the breast tissue of hyperplasia ratswith mammary gland diseases. Methods The unfertilized female Wistar rats were selected,8 rats was selected as normal controlgroup,the rest of the rats were given muscle injection of estradiol benzoate (0.5 mg/kg) every day,25 d in a row,then every rat wasgiven muscle injection of progesterone (4 mg/kg) one time to copy the mammary gland hyperplasia rats model,and normal controlgroup rats was given muscle injection of saline solution. After successful model replication,the model rats were randomly divided intomodel control,positive control,low-dose and high-dose M-Ⅰ groups. The pathological features of the breast tissue of rats were ob-served,and the expressions of Prdx-1,GSTP and SOD in the serum and breast tissue were detected.Results Compared with those ofnormal control group,the Prdx-1 and GSTP expressions in breast tissue of model control group were significantly increased (P<0.01 or P<0.05),the SOD expression was significantly decreased (P<0.05),and hyperplasia of breast tissue was significantly changed. Com-pared with those of model group,the Prdx-1 expression in breast tissue in low-dose and high-dose M-Ⅰ groups was significantly re-duced,and the expressions of GSTP and SOD were significantly increased (P < 0.01 or P < 0.05). Conclusions M-Ⅰ has a goodtherapeutic effect on hyperplasia rats,and its therapeutic mechanism involves the regulation of Prdx-1,GSTP and SOD.更多还原