【摘要】 目的探讨20-羟二十烷四烯酸(20-HETE)对人胎盘绒毛膜滋养细胞生物学行为的影响。方法体外培养人胎盘绒毛膜滋养细胞,分成正常对照组、药物介质组、20-HETE组、HET0016组,分别加入细胞培养基、二甲基亚砜、20-HETE及20-HETE抑制剂HET0016作用48h;采用ELISA法检测人胎盘绒毛膜滋养细胞基质金属蛋白酶-9(MMP-9)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)浓度,RT-PCR法检测人胎盘绒毛膜滋养细胞MMP-9、NGAL m RNA表达。结果与正常对照组比较,药物介质组人胎盘绒毛膜滋养细胞MMP-9、NGAL浓度及m RNA相对表达量差异均无统计学意义(均P>0.05),20-HETE组人胎盘绒毛膜滋养细胞MMP-9、NGAL浓度及m RNA相对表达量均明显降低(均P<0.05),HET0016组人胎盘绒毛膜滋养细胞MMP-9、NGAL浓度及m RNA相对表达量均明显升高(均P<0.05)。结论 20-HETE可抑制人胎盘绒毛膜滋养细胞MMP-9、NGAL浓度及m RNA表达,而其抑制剂HET0016起促进作用。更多还原

【Abstract】 Objective To investigate the effect of 20-hydroxyeicosatetraenoic acid(20-HETE) on the biological behavior of human placental choriotrophoblasts.Methods Human placental choriotrophoblasts were cultured in vitro and divided into normal control group,drug-mediated group,20-HETE group and HET0016 group,which were treated with cell culture medium,dimethyl sulfoxide,20-HETE and20-HETE inhibitor HET0016 for 48 h,respectively.The protein concentrations and m RNA expression of matrix metalloproteinase-9(MMP-9)and neutrophil gelatinase-associated lipocalin(NGAL) in the supernatant of human placental choriotrophoblasts were detected by ELISA and RT-PCR,respectively.Results There were no significant difference in protein concentrations and m RNA expressions of MMP-9 and NGAL in human placental choriotrophoblasts between drug-mediated group and normal control group(P >0.05),while the protein concentration and m RNA expressions in the 20-HETE group were significantly lower,and in the HET0016 group were significantly higher than those in the normal control group(P <0.05).Conclusion 20-HETE can inhibit the concentrations and the m RNA expressions of MMP-9 and NGAL in human placental choriotrophoblasts,and its inhibitor HET0016 can reverse the effects.更多还原