【摘要】 目的:探讨针刺对血管性痴呆(VD)大鼠脑组织中引起细胞凋亡相关蛋白Caspase-3、NFK-βp65及PTEN mRNA表达的影响,为临床治疗血管性痴呆提供理论依据。方法:将水迷宫测试合格的大鼠随机分5组(正常组、假手术组、模型组、手针组、电针组,每组24只)。运用四血管阻断法制作VD大鼠模型,造模成功7天后,针刺大鼠头穴干预。通过Morris水迷宫实验测试大鼠的学习记忆能力;免疫组化检测海马Caspase-3、NFK-βp65阳性表达。结果:相同时刻,针刺组(手针组、电针组)大鼠与模型组大鼠对比,成功穿越平台次数增多,逃避潜伏期缩短,差异有统计学意义(P <0. 05或P <0. 01);同一时间点,电针组大鼠较手针组穿越平台成功次数明显增多,差异有统计学意义(P <0. 05)。手针组和电针组治疗第7天、第14天、第21天3个时间点与同一时点模型组Caspase-3阳性细胞表达相比有所下降,均具有显著性差异(P <0. 05或P <0. 01),说明手针治疗和电针治疗均能降低Caspase-3阳性细胞表达。手针组和电针组治疗第7天、第14天、第21天3个时间点与同一时点模型组NFK-βp65阳性细胞表达相比有所下降,均具有显著性差异(P <0. 05或P <0. 01),说明手针治疗和电针治疗均能降低NFK-βp65阳性细胞表达。手针组和电针组治疗第7天、第14天、第21天3个时间点与同一时点模型组PTEN mRNA表达相比有所下降,均具有显著性差异(P <0. 01),说明手针治疗和电针治疗均能降低PTEN mRNA表达。结论:针刺具有明显抑制血管性痴呆(VD)大鼠脑组织细胞凋亡相关蛋白Caspase-3、NFK-βp65及PTEN mRNA表达的影响,具有脑保护作用。更多还原

【Abstract】 Objective: To investigate the effect of acupuncture on the expressions of apoptosis-related proteins( caspase-3 and NFK-βp65) and PTEN mRNA in brain tissue of the rats with vascular dementia( VD),thus to provide theoretical basis for the clinical treatment of VD. Methods: 168 rats that passed the water maze test were randomly divided into five groups( the normal group,the sham operation group,the model group,the manual needling group,the electro-acupuncture group,24 rats in each group). The model of VD rats was made by four-blood vessel block method. 7 days after successful modeling,scalp needling intervention was applied. The learning ability and memory ability of the rats were measured by Morris water maze. The expressions of Caspase-3 and NFK-βp65 in hippocampus were detected by immunohistochemistry. Results: At the same time point,evasive incubation period was shortened,and the number of crossing platform was increased in the manual needling group and in the electro-acupuncture group compared to those in the model group( P < 0. 05,P < 0. 01); the number of crossing platform was significantly increased in the electro-acupuncture group compared to that in the the manual needling group( P < 0. 05). On 7 th day,14 th day,and 21 st day of the treatment,the expressions of Caspase-3 positive cells,NFK-βp65 positive cells,and PTEN mRNA were significantly reduced in the manual needling group and in the electro-acupuncture group compared to those in the model group( P < 0. 05,P < 0. 01). Conclusion: Acupuncture can effectively inhibit the expressions of caspase-3,NFK-βp65 and PTEN mRNA in brain tissue of rats with VD. It has brain protection.更多还原