Neural Circuits Basis of Temporal Lobe Epilepsy

【摘要】 Temporal lobe epilepsy(TLE) is a common type of epilepsy and not well controlled by current treatments,but the underlying cellular/circuit mechanisms remain unclear. The early series of our studies have proved the success of low-frequency stimulation treatment for epilepsy,which was mainly depending on the stimulation target,the stimulation frequency and stimulation time(the therapeutic-window phenomenon). Now,by using optogenetics,multiple-channel EEG analysis,imaging,electrophysiological and molecular techniques,we are continued to investigate the circuit mechanism of therapeutic deep brain stimulation,and found that entorhinal principal neurons mediate antiepileptic "glutamatergic-GABAergic" neuronal circuit for brain stimulation treatments of epilepsy. Meanwhile,we are currently focusing on the interplay of inhibitory and excitatory network in subicular microcircuits especially that related to the generation of generalized seizures(GS) in TLE,and we found that depolarized GABAergic signaling in subicular microcircuit mediates GS in TLE. This may be of therapeutic interest in understanding the pathological neuronal circuitry and further the development of novel therapeutic approaches.更多还原

【Abstract】 Temporal lobe epilepsy(TLE) is a common type of epilepsy and not well controlled by current treatments,but the underlying cellular/circuit mechanisms remain unclear. The early series of our studies have proved the success of low-frequency stimulation treatment for epilepsy,which was mainly depending on the stimulation target,the stimulation frequency and stimulation time(the therapeutic-window phenomenon). Now,by using optogenetics,multiple-channel EEG analysis,imaging,electrophysiological and molecular techniques,we are continued to investigate the circuit mechanism of therapeutic deep brain stimulation,and found that entorhinal principal neurons mediate antiepileptic "glutamatergic-GABAergic" neuronal circuit for brain stimulation treatments of epilepsy. Meanwhile,we are currently focusing on the interplay of inhibitory and excitatory network in subicular microcircuits especially that related to the generation of generalized seizures(GS) in TLE,and we found that depolarized GABAergic signaling in subicular microcircuit mediates GS in TLE. This may be of therapeutic interest in understanding the pathological neuronal circuitry and further the development of novel therapeutic approaches.更多还原