【摘要】 采用荧光原位杂交（Fluorescence in Situ Hybridization,FISH）技术分析小伞山羊草染色体的核型特点。结果表明,小伞山羊草共包含7对染色体,其中1 U和5 U染色体各含有1对随体。分别以Oligo-pSc 119.2-1（绿色）与（GAA）₇（红色）重复序列为探针对小伞山羊草根尖细胞染色体进行FISH分析,发现Oligo-pSc-119.2-1信号主要分布在染色体的端部及近端部,不同染色体之间的信号强度有所差别。（GAA）₇信号主要集中于染色体着丝点附近,不但比Oligo-pSc-119.2-1信号的亮度高,分布丰富,而且在染色体上的分布可与Oligo-pSc-119.2-1信号互补。因此,同时采用Oligo-pSc-119.2-1与（GAA）₇ 2种探针能准确地辨别小伞山羊草的每对染色体,建立了小伞山羊草的FISH核型。更多还原

【Abstract】 FISH（Fluorescence in situ hybridization） technique was applied to analyze chromosome patterns of Aegilops umbellulata.The results showed that Aegilops umbellulata contained a total of 7 pairs of chromosomes, of which 1 U and 5 U chromosomes had a pair of satellites, respectively.Using the Oligo-pSc 119.2-1（green） and（GAA）₇（red） repetitive sequence as probes,chromosome FISH analysis of the root tip cells in Aegilops umbellulata showed that the Oligo-pSc-119.2-1 signals were mainly distributed in the telomeres and proximal telomeres of chromosomes,and the signal intensity varied among different chromosomes.The（GAA）₇ signals were generally concentrated near the centromeres of the chromosomes,which were not only more luminous and abundant than those of Oligo-pSc-119.2-1 signals,but also complementary distribution to those of Oligo-pSc-119.2-1 signals on chromosomes.Therefore, both Oligo-pSc 119.2-1 and（GAA）₇ probes were used to accurately identify each pair of chromosomes in Aegilops umbellulata,and its FISH karyotype was established.更多还原