【摘要】 目的探究CD151对结直肠癌(CRC)血管增生的影响及其机制。方法通过RealTime PCR和Western blot技术检测HT-29和CD151--HT29细胞中CD151和β-catenin的表达情况;通过裸鼠皮下成瘤实验,采用免疫组织化学CD34标记血管内皮细胞的方法观察HT-29和CD151--HT29两组裸鼠皮下成瘤情况和移植瘤血管生成的差异。结果 CD151--HT29组细胞中CD151和β-catenin表达明显低于对照组(P <0.05);CD151--HT29组裸鼠皮下瘤重量和微血管密度(MVD)明显低于对照组(P <0.05)。结论 CD151可通过上调β-catenin蛋白的表达来促进CRC血管增生。更多还原

【Abstract】 Objective To explore the effect and mechanism of CD151 on angiogenesis in colorectal cancer(CRC). Methods The expression of CD151 and β-catenin were detected by Western blot and RT-qPCR in HT-29 and CD151--HT29 cells. The microvessel density of the transplanted tumor tissues was observed by immunohistochemistry CD34 labeled vascular endothelial cells. Results The expression of CD151 and β-catenin were lower in CD151--HT29 cells compared with HT29 cells(P<0.05). The microvessel density(MVD) count of the CD151--HT29 group was significantly lower than that of the HT-29 group(P < 0.05). Conclusion CD151 could promote angiogenesis via up-regulate β-catenin in CRC.更多还原