【摘要】 【目的】为明确辽宁省260份粳稻品种中稻瘟病抗性基因的类型,评价抗性基因利用价值,为辽宁省水稻抗病育种提供参考。【方法】选用9个稻瘟病抗性基因的特异引物分析辽宁省已审定的260份粳稻品种抗性基因种类、基因组合类型及地域分布情况,基于抗病基因检测条带的有无进行品种聚类分析,苗期人工喷雾接种、分蘖盛期和蜡熟期田间自然发病调查,评价9个抗性单基因系的抗病性。【结果】辽宁省粳稻品种中含有抗性基因数量从0～7个不等,抗性基因Pikh检出率最高,其次是Pikm和Pi1;所有参试品种均未检测到Pi9基因,仅在辽宁省农业科学院育成的辽粳421中检测到Pi40基因。参试的水稻品种中共有62个基因组合类型。基于抗性基因检测结果进行聚类分析,可以将260份粳稻材料分为11个类群。9个抗性单基因系中,Pi40抗性最好;其次是Pi9、Pigm和Pita。【结论】抗性基因Pi40、Pi9、Pigm和Pita在辽宁省粳稻品种选育和生产上具有重要的应用潜力;参试品种的80.38%集中在第Ⅴ、Ⅵ、Ⅶ和Ⅸ类群,没有明显的地域差异;参试品种抗病基因组合类型不够丰富,亲缘关系较近。更多还原

【Abstract】 【Objective】To clarify the rice blast resistance genes in 260 japonica rice varieties in Liaoning Province,evaluate their utilization value, and provide reference for rice breeding,【Method】specific primers of nine rice blast resistance genes were selected to analyze the species, the quantity and the geographical distribution of resistance genes of260 japonica rice varieties in Liaoning, and the cluster analysis of varieties was based on the detection bands of disease resistance genes. The disease resistance of the nine resistant mongenic rice lines was evaluated by seedling-spray inoculation, natural disease investigation during the active tillering stage and ripening stage.【Result】The number of blast resistance genes in japonica rice from Liaoning varied from 0 to 7, and the frequency of Pikh gene ranked highest,followed by the Pikm and Pi1. Pi9 gene was not detected in all tested varieties, and Pi40 gene was only detected in Liaojing 421 bred by the Liaoning Academy of Agricultural Sciences. There were 62 gene combinations in the rice varieties tested, and based on the clustering analysis of the results of the resistance gene detection, 260 japonica rice materials can be divided into 11 groups. Among the nine resistant mongenic lines, Pi40 showed the best resistance,followed by Pi9, Pigm and Pita.【Conclusion】The resistance genes Pi9, Pi40, Pita, and Pigm have important application potential in breeding in Liaoning Province. 80.38% of the tested varieties concentrate in groups V, VI, VII and IX. There is no obvious geographical difference; the types of disease resistance gene combinations of the tested varieties are relatively less abundant and the genetic relationship is relatively close.更多还原