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12周龄大围山微型鸡与艾维茵肉鸡肌肉营养成分及LPL基因表达差异研究

Study on the Differences of Muscle Nutrient Composition and LPL Gene Expression in Daweishan Mini Chicken and Avian Broiler at 12 Weeks of Age

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【作者】 柳明正; 徐志强; 豆腾飞; 王坤; 刘丽仙; 谷大海; 程志斌; 曹振辉; 黄英; 李琦华; 贾俊静; 葛长荣;

【Author】 LIU Mingzheng;XU Zhiqiang;DOU Tengfei;WANG Kun;LIU Lixian;GU Dahai;CHEN Zhibin;CAO Zhenhui;HUANG Ying;LI Qihua;JIA Junjing;GE Changrong;Faculty of Animal Science and Technology, Yunnan Provincial Key Laboratory ofAnimal Nutrition and Feed, Yunnan Agricultural University;College of Food Science and Technology,Yunnan Agricultural University;

【通讯作者】 葛长荣;

【机构】 云南农业大学动物科学技术学院云南省动物营养与饲料重点实验室; 云南农业大学食品科学技术学院;

【摘要】 为研究云南地方鸡种大围山微型鸡与艾维茵肉鸡肌肉营养成分中粗脂肪含量与脂蛋白脂酶(LPL)基因mRNA表达差异,屠宰同批12周龄大围山微型鸡和艾维茵肉鸡各20只,测定肌肉营养成分含量和LPL基因表达量。结果显示:在胸肌和腿肌中,大围山微型鸡粗灰分含量、粗蛋白含量、粗脂肪含量均显著高于艾维茵肉鸡,在胸肌和腿肌中大围山微型鸡水分的含量显著低于艾维茵肉鸡,且胸肌和腿肌中大围山微型鸡LPL基因表达量分别为3.98、5.72,均高于艾维茵肉鸡的3.70、3.54。可见,大围山微型鸡肌肉粗蛋白、粗脂肪等营养成分优于艾维茵肉鸡,LPL基因促进脂肪沉积,因此LPL基因可作为影响家禽脂肪沉积的重要候选基因。

【Abstract】 In order to study the differences of muscle nutrient composition and LPL gene expression in Daweishan mini chicken and Avian broiler, 20 Daweishan mini chickens and 20 Avian broilers were slaughtered at 12 weeks of age, and muscle nutrient content and LPL gene expression were measured. The results showed that, in breast muscle and leg muscle the crude ash content, crude protein content and crude fat content of Daweishan mini chickens were significantly higher than that of Avian broilers. However, the moisture content of Daweishan mini chickens in breast muscle and leg muscle were significantly lower than that of Avian broilers. The LPL gene expression in breast and leg muscle of Daweishan mini chickens was 3.98 and 5.72, both higher than that of Avian broilers(3.70 and 3.54). Daweishan mini chicken showed better muscle crude protein, crude fat and other nutrients than Avian broiler, and LPL gene could promote fat deposition in muscle. So LPL gene can be used as an important candidate gene to affect fat deposition in poultry.

【基金】 国家自然科学基金项目(U1702232、31560618、31572359);云南省自然科学基金重点项目(2016FA046);云南省科技厅南希·莱恩院士工作站(2017IC048);云岭学者及云岭产业技术领军人才培养经费
  • 【分类号】S831.2
  • 【被引频次】6
  • 【下载频次】188
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