【摘要】 从垃圾渗滤液中分离得到了一株亚硝化单胞菌Nitrosomonas eutropha CZ-4,其16S rDNA序列与N. eutropha C91的相似性达99%.研究了pH值、温度、游离亚硝酸浓度、盐度等对其生长的影响,并测试了其在垃圾渗滤液、黑臭水和富营养化湖水中的脱氨效果.结果表明,该菌的最适生长pH值为7.3～8.7,最适生长温度为30.9℃,游离亚硝酸和盐度对该菌的半数抑制浓度分别约为0.11mg/L与2%.在最佳发酵条件下,该菌的最大氨氮去除速率为58mg/(L·h),最短倍增时间为8.2h;在不同类型的污水/地表水(初始氨氮浓度为0.66～603mg/L)中,该菌的最大氨氮去除速率为11.4mg/(L·h),最短倍增时间为10.9h,最低残余氨氮浓度为0.11mg/L.更多还原

【Abstract】 A Nitrosomonas strain was isolated from the landfill leachate. It was named as Nitrosomonas eutropha CZ-4 for its 16 s rDNA sequence was highly similar to N. eutropha C91 at an identity of 99%. This work studied the influences of pH, temperature, free nitrous acid concentration and salinity on the growth of N. eutropha CZ-4, as well as its NH₃-N removal abilities in landfill leachate, black odor water and eutrophic lake water. The results showed that the optimum growth pH of the strain was 7.3～8.7, the optimum growth temperature was 30.9°C, and the IC50 of free nitrous acid and salinity was about 0.11 mg/L and 2%（in terms of NaCl）, respectively. Under the optimal fermentation condition, the maximum NH₃-N removal rate reached 58 mg/（L·h）, and the shortest doubling time was 8.2 h. In different types of sewage/surface water（their initial ammonia concentration ranged from 0.66 mg/L to 603 mg/L）, the NH₃-N removal rate was up to 11.4 mg/（L·h）, the doubling time was as short as 10.9 h, and the residual NH₃-N concentration was down to 0.11 mg/L.更多还原