【摘要】 利用Illumina MiSeq对sc CO₂-咸水-砂岩体系中微生物16S rRNA基因V3-V4区进行分析,探究高压反应釜体系中微生物对sc CO₂的响应及微生物对矿物反应的作用.结果显示,生物量受pH值影响较大,初始pH值为7.02,生物量11.02×10⁶gene/mL,30d时pH值降至5.65,生物量降至0.26×10⁶gene/mL;随着矿物溶蚀,90d时pH值增至5.87,生物量增至4.61×10⁶gene/mL.群落结构中,phylum Proteobacteria（52.60%（30d）,55.34%（90d））与phylum Firmicutes（46.89%（30d）,43.89%（90d））为优势菌门.在属水平,30,90d时Exiguobacterium,Citrobacter,Acinetobacter与Pseudomonas为优势菌属.产酸菌（Exiguobacterium,Acinetobacter与Pseudomonas）促进了长石与粘土溶蚀,咸水中K⁺,Na⁺,Ca²⁺,Mg²⁺,T-Fe浓度高于空白组;铁还原菌（Citrobacter）提高了Fe（Ⅱ）/Fe（Ⅲ）比值;微生物膜对Ca²⁺?Mg²⁺?Fe²⁺具有吸附作用.SEM结果显示,微生物介导下先于空白组出现菱铁矿沉淀. scCO₂^-咸水-砂岩体系中适应菌能促进矿物溶蚀与碳酸盐矿物捕获.更多还原

【Abstract】 Through the Illumina MiSeq analysis within V3-V4 region of 16 S rRNA gene, the response of microorganisms to scCO2 and its feedback on the mineral interaction in scCO₂^-saline-sandstone system was investigated. The results showed that biomass was affected by p H value. The initial p H was 7.02 and biomass was 11.02×10⁶gene/mL. And the pH dropped to 5.65 and biomass decreased to 0.26×10⁶gene/mL at 30th-day. However with the dissolution of minerals, the pH increased to 5.87 and biomass increased to 4.61×10⁶gene/mL at 90th-day. In the community structure, phylum Proteobacteria（52.60%（30d）, 55.34%（90d）） and phylum Firmicutes（46.89%（30d）, 43.89%（90d）） were dominant phylum. At the genus level, Exiguobacterium, Citrobacter, Acinetobacter and Pseudomonas were dominant genus at 30 th and 90th-day. Acid-producing bacteria（Exiguobacterium, Acinetobactera and Pseudomonas） promoted the dissolution of feldspar and clay, and the concentrations of K⁺, Na⁺, Ca²⁺, Mg²⁺ and T-Fe were higher than those in blank group. Iron-reducing bacteria（Citrobacter） increased the ratio of Fe（Ⅱ）/Fe（Ⅲ）. Biofilm showed an adsorption function of Ca²⁺, Mg²⁺ and Fe²⁺. At last, the SEM results showed that the micron-mediated precipitation of siderite appeared before the blank group. Therefore, the adaptable bacteria in the scCO₂^-salt-sandstone system could promote the mineral dissolution and carbonates capture.更多还原