【摘要】 目的对毛茛科铁线莲属植物女萎Clematis apiifolia的化学成分进行研究。方法通过硅胶、ODS、凝胶Sephadex LH-20、HW-40C等多种色谱分离技术分离和纯化,利用核磁共振(NMR)及质谱等现代波谱学技术鉴定化合物结构。结果从女萎乙醇提取物中分离得到20个化合物,分别鉴定为常春藤皂苷元(1)、α-hederin(2)、sapindosideB(3)、豆甾-22-烯-3β,6β,9β-三醇(4)、pleuchiol(5)、豆甾醇-3-O-β-D-葡萄糖苷(6)、豆甾醇(7)、毒马草素(8)、莽草酸(9)、乙基-α-D-葡萄糖苷(10)、乙基-β-D-葡萄糖苷(11)、D-葡萄糖醛酸(12)、3,5-二羟基-4-戊内酯(13)、2-醛基-5-羟甲基呋喃(14)、尿嘧啶(15)、尿嘧啶核苷(16)、正二十三烷醇(17)、正三十烷醇(18)、正二十六烷酸(19)、棕榈酸-1-甘油酯(20)。结论所有化合物均为首次从女萎中分离鉴定,其中化合物3为首次从铁线莲属植物中分离得到,化合物4、5、9～12为首次从毛茛科植物中分离得到。更多还原

【Abstract】 Objective To investigate the chemical constituents from the stem of the Clematis apiifolia. Methods A variety of chromatographic methods were applied in isolation and purification including silica gel, ODS, Sephadex LH-20 gel, and HW-40 C and so on. Their structures were identified by the nuclear magnetic resonance(NMR), mass spectrometry and so on. Results These compounds were isolated and determined as hederagenin(1), α-hederin(2), sapindoside B(3), stigmast-22-en-3β, 6β, 9β-triol(4), pleuchiol(5), stigmasterol-3-O-β-D-glucopyranoside(6), stimasterol(7), siderin(8), shikimic acid(9), ethyl-α-D-glucopyranoside(10), ethyl-β-D-glucopyranoside(11), D-gulonic acid(12), 3,5-dihydroxyl-4-valerolactone(13), 2-formyl-5-hydroxymethylfuran(14), uracil(15), uridine(16), tricosanol(17), triacontanol(18), hexacosanoic acid(19), and monostearin(20). Conclusion All compounds are obtained from C. apiifolia for the first time including firstly reported compound 3 from Clematis plants and compounds 4, 5, 9—12 from Ranunculaceae family.更多还原