【摘要】 目的:探讨白细胞介素17(IL-17)对人子宫内膜癌细胞的活力、迁移和侵袭能力的影响及其潜在作用机制。方法:收集人子宫内膜癌组织和良性子宫病变组织,RT-qPCR法检测组织中IL-17和微小RNA-195-5p(miR-195-5p)的表达。以不同浓度IL-17干预人子宫内膜癌HEC-1-B细胞48 h,RT-qPCR法检测细胞中miR-195-5p表达。采用MTT和Transwell实验分别检测100μg/L IL-17干预或转染miR-195-5p mimics后HEC-1-B细胞的活力、迁移能力和侵袭能力改变;过表达miR-195-5p联合100μg/L IL-17干预,检测HEC-1-B细胞的活力、迁移能力和侵袭能力。结果:在人子宫内膜癌组织中,IL-17高表达而miR-195-5p低表达(P<0.05)。浓度为10、100和300μg/L的IL-17干预HEC-1-B细胞48 h后,miR-195-5p的表达水平显著降低。MTT和Transwell实验结果表明,100μg/L IL-17能够提高HEC-1-B细胞的活力、迁移能力和侵袭能力(P<0.05),而过表达miR-195-5p所得结果相反(P<0.05)。过表达miR-195-5p能够减弱IL-17对HEC-1-B细胞活力、侵袭和迁移的促进作用(P<0.05)。结论:IL-17在人子宫内膜癌组织中高表达。外源性IL-17能够促进人子宫内膜癌HEC-1-B细胞的生长、迁移和侵袭,这一作用可能与抑制miR-195-5p表达有关。更多还原

【Abstract】 AIM: To investigate the potential mechanism of interleukin-17(IL-17) promoting the viability, migration and invasion of human endometrial carcinoma cells. METHODS: The expression of IL-17 and microRNA-195-5 p(miR-195-5 p) in the human endometrial carcinoma and benign uterine lesion samples were detected by RT-qPCR. The expression of miR-195-5 p in human endometrial carcinoma HEC-1-B cells after treatment with IL-17 at different concentrations for 48 h was detected by RT-qPCR. The viability, migration and invasion of HEC-1-B cells after treatment with IL-17 at 100 μg/L or transfection of miR-195-5 p mimics were detected by MTT assay and Transwell assays. The viability, migration and invasion of HEC-1-B cells after over-expression of miR-195-5 p combined with 100 μg/L IL-17 intervention were also observed. RESULTS: The expression of IL-17 was increased while the expression of miR-195-5 p was decreased in the human endometrial carcinoma samples(P<0.05). The expression of miR-195-5 p in the HEC-1-B cells after treatment with IL-17 at 10,100 and 300 μg/L for 48 h was significantly decreased(P<0.05). The results of MTT assay and Transwell experiments indicated that IL-17 at 100 μg/L enhanced the viability, migration and invasion of HEC-1-B cells, while over-expression of miR-195-5 p resulted in the opposite effect. CONCLUSION: Over-expression of miR-195-5 p inhibits the enhancing effects of IL-17 on the viability, invasion and migration of HEC-1-B cells.更多还原