【摘要】 目的探讨胡萝卜结合态多酚(bound polyphenol in Daucus carota,BPDC)对体外培养的Hep G-2肝癌细胞增殖抑制和促进凋亡的作用。方法 MTT法观察BPDC对Hep G-2细胞增殖抑制作用;光学显微镜观察BPDC作用后肝癌细胞的形态学变化;流式细胞术检测BPDC对Hep G-2肝癌细胞的凋亡作用,以及对Hep G-2细胞周期和线粒体膜电位的影响;利用各种试剂盒检测BPDC作用下Hep G-2细胞凋亡相关蛋白Caspase-3、Caspase-8、Caspase-9的酶活性变化。结果不同浓度的BPDC(0.05、0.1、0.15、0.2、0.3、0.4、0.5 mg/ml)处理的Hep G-2肝癌细胞的总抑制率分别为14.9%、48.1%、56.0%、64.0%、71.9%、73.8%、74.2%,与对照组相比,均达到了极显著差异;BPDC处理24小时的细胞周期检测结果显示Hep G-2细胞被阻滞在G2期;BPDC处理12小时的细胞凋亡检测结果显示,对照组、低浓度组(0.10 mg/ml)和高浓度组(0.3 mg/ml)的Hep G-2早期凋亡细胞比例分别为0.83%、27.75%和62.07%;细胞形态、细胞凋亡检测结果均显示,BPDC能够显著促进Hep G-2细胞的凋亡;线粒体膜电位、Caspase等生理、生化指标检测结果显示BPDC能够诱导Hep G-2细胞进入程序性凋亡。结论 BPDC能够显著抑制Hep G-2细胞的增殖,诱导和促进Hep G-2细胞的凋亡;凋亡机制与激活外部途径(extrinsicpathway)和线粒体途径(mitochondrialpathway)有关。[营养学报,2019,41(4):386-392,397]更多还原

【Abstract】 Objective To investigate the effects of the bound polyphenols from Daucus carota(BPDC) on proliferation and apoptosis of HepG2 cells. Methods MTT was used to assess cell proliferation. The morphological changes of HepG2 cells after BPDC treatment were observed under an optical microscope. Cell apoptosis, cell cycle, and mitochondrial membrane potential were detected by flow cytometry. The activity levels of apoptosis-related proteins such as caspase-3, caspase-8, and caspase-9 were evaluated by corresponding test kits. Results The inhibition rates of HepG2 cells were 14.9%, 48.1%, 56.0%, 64.0%, 71.9%, 73.8% and 74.2%, respectively in response to different concentrations of BPDCs(0.05, 0.1, 0.15, 0.2, 0.3, 0.4 and 0.5 mg/ml) treatment. Compared with the control group, all inhibition rates reached a significant level. The cell cycle detection showed that HepG2 cells were blocked in the G2 phase after BPDC treatment for 24 hours. The apoptosis test showed that the proportion of early apoptotic cells were 0.83%, 27.75% and 62.07%, respectively after HepG2 cells were treated with different concentrations of BPDCs(0, 0.1, 0.3 mg/ml) for 12 hour. The changes of mitochondrial membrane potential, caspase activities in HepG2 cells showed that BPDC could induce the development of the apoptosis. Conclusion BPDC can significantly inhibit the proliferation of HepG2 cells, and induce the apoptosis of HepG-2 cells by activating the extrinsic and mitochondrial pathways.[ACTA NUTRIMENTA SINICA, 2019, 41(4):386-392,397]更多还原