【摘要】 从‘嘎拉’苹果(Malus×domestica Borkh.)中克隆蔗糖转运蛋白基因MdSUT2(序列号:MDP0000277235),该基因包含1 704 bp开放阅读框,编码566个氨基酸,蛋白大小为56 kD。构建MdSUT2表达载体并利用农杆菌介导法侵染拟南芥,半定量RT-PCR证明Md SUT2在拟南芥中异源表达。构建瞬时表达载体,‘红星’苹果进行Md SUT2瞬时表达,其花青苷含量增多,而沉默该基因花青苷含量明显降低。更多还原

【Abstract】 A gene named MdSUT2(MDP0000277235)was cloned from‘Gala’apple(Malus × domestica Borkh.). Sequence analysis showed that this gene contained 1 704 bp nucleotides,and encoded a protein of 566 amino acids. Overexpression vector of MdSUT2 was constructed and transformed into Arabidopsis seedlings via Agrobacterium-mediated method. Semi RT-PCR analysis indicated that MdSUT2 was genetically transformed into Arabidopsis. In addition, the transient expression vectors were constructed and transformed into apple fruits. We found that overexpression of MdSUT2 in apple fruits significantly increased the anthocyanin content, whereas suppression of MdSUT2 decreased the anthocyanin content.更多还原