【摘要】 目的探讨黄芪甲苷对过氧化氢（H₂O₂）损伤模型人脐静脉内皮细胞（HUVEC）的改善作用。方法将对数生长期的HUVEC分为正常对照组（A组,常规培养基）,H₂O₂组（B组,300μmol/L H₂O₂）,阳性对照组（C组,300μmol/L H₂O₂+50μg/m L维生素E）,以及黄芪甲苷高、中、低剂量组（D₁组、D₂组、D₃组,300μmol/L H₂O₂+50. 0,25. 0,12. 5μg/m L黄芪甲苷）。采用四氮唑盐（MTT）法测定细胞活力,测定乳酸脱氢酶（LDH）及超氧化物歧化酶（SOD）活性,采用实时荧光定量聚合酶链式反应（RT-PCR）法测定单核细胞趋化蛋白1（MCP-1） mRNA及细胞间黏附分子1（ICAM-1） mRNA表达情况。结果与A组比较,B组细胞活力及SOD活性显著减弱,LDH活性及MCP-1 mRNA,ICAM-1 mRNA表达显著增强（P <0. 01）;与B组比较,C组及D₁组、D₂组、D₃组细胞活力及SOD活性显著增强,LDH活性及MCP-1 mRNA和ICAM-1 mRNA表达显著减弱（P <0. 01）。结论黄芪甲苷对H₂O₂损伤HUVEC有一定改善作用,其机制可能与减弱MCP-1 mRNA和ICAM-1 mRNA表达有关。更多还原

【Abstract】 Objective To investigate the improvement effect of astragaloside Ⅳ on the human umbilical vein endothelial cell（ HUVEC）injured by hydrogen peroxide（ H₂O₂）. Methods The HUVEC in logarithmic growth phase were divided into the normal control group（ group A,conventional medium）,H₂O₂ group（ group B,300 μmol/L H₂O₂）,positive control group（ group C,300 μmol/L H₂O₂ + 50 μg/m L vitamin E） and astragaloside Ⅳ high,medium and low dosage groups（ group D₁,group D₂,group D₃,300 μmol/L H₂O₂ + 50. 0,25. 0,12. 5 μg/m L astragaloside）. The cell viability was measured by methyl thiazolyl tetrazolium（ MTT） assay,the LDH and SOD activities were measured by biochemical detection,the monocyte chemoattractant protein-1（ MCP-1） mRNA and intercellular adhesion molecule-1（ ICAM-1） mRNA were measured by real-time fluorescence quantitative polymerase chain reaction（ RT-PCR）. Results Compared with those in group A,the cell viability and SOD activity were significantly decreased,while the LDH activity,MCP-1 mRNA and ICAM-1 mRNA expression were significantly increased in group B（ P < 0. 01）. Compared with those in group B,the cell viability and SOD activity were significantly increased,while the LDH activity,MCP-1 mRNA and ICAM-1 mRNA expression were significantly decreased in group C,group D₁,group D₂ and group D₃（ P < 0. 01）. Conclusion Astragaloside Ⅳ can improve H₂O₂-induced HUVEC damage,and its mechanism may be related to the decrease of MCP-1 and ICAM-1 expression.更多还原