【摘要】 目的探讨参附注射液对脓毒症小鼠免疫调节及炎症因子的影响。方法选取120只10周龄SPF级雄性C57BL/6小鼠,按随机数字表法将其分为对照组、假手术组、脓毒症模型组、参附注射液治疗组,每组30只。采用盲肠结扎穿孔法制备脓毒症小鼠模型。对照组、假手术组和脓毒症模型组于术后第1天开始腹腔注射生理盐水[4 mL/(kg·BW),共3 d],参附注射液治疗组腹腔注射参附注射液[4 mL/(kg·BW),共3 d]。采用酶联免疫吸附实验检测各组小鼠T淋巴细胞亚群;采用苏木精-伊红染色法评价器官病理损伤及炎症细胞浸润情况。结果与对照组比较,假手术组白细胞介素-4(IL-4)、IL-17、γ干扰素(IFN-γ)、IL-10水平及IFN-γ/IL-4、IL-10/IL-17差异无统计学意义(P> 0.05)。与假手术组比较,脓毒症模型组IFN-γ、IL-10水平均显著降低,IL-4和IL-17水平显著升高,IFN-γ/IL-4、IL-10/IL-17均显著降低,差异均有统计学意义(P <0.05);其心脏、肺、肾脏病理损伤严重,炎症细胞浸润均增加(P <0.05);与脓毒症模型组比较,参附注射液治疗组IFN-γ、IL-10水平均升高,IL-4、IL-17水平均降低,IFN-γ/IL-4、IL-10/IL-17均升高,但低于对照组,差异均有统计学意义(均P <0.05);其器官病理损伤明显缓解,炎症细胞浸润情况减轻(P <0.05)。结论参附注射液能够调节脓毒症小鼠的免疫系统,抑制炎性反应,减轻器官损伤。更多还原

【Abstract】 Objective To investigate the effects of Shenfu Injection on immuno regulation and inflammatory factors in septic mice. Methods A total of 120 10-week-old SPF male C57BL/6 mice were selected and divided into control group, sham operation group, septic model group and Shenfu Injection treatment group according to random number table method, with 30 rats in each group. A septic mice model was prepared by cecal ligation and perforation. The control group, sham-operated group and sepsis model group were given intraperitoneal injection of saline [4 mL/(kg·BW)for 3 days] respectively on the first day after operation, and Shenfu Injection group was given intraperitoneal injection of Shenfu Injection [4 mL/(kg·BW) for 3 days] at the same time. T lymphocyte subsets in each group were detected by enzyme-linked immunosorbent assay, hematoxylin-eosin staining was used to evaluate organ pathological damage and inflammatory cell infiltration. Results Compared with the control group, the levels of interleukin-4(IL-4), IL-17, interferon γ(IFN-γ), IL-10 and IFN-γ/IL-4, IL-10/IL-17 in sham operation group had no significant difference(P >0.05). Compared with sham-operated group, the levels of IFN-γ and IL-10 in sepsis model group were significantly decreased, IL-4 and IL-17 were significantly increased, and IFN-γ/IL-4 and IL-10/IL-17 were significantly decreased, the differences were statistically significant(P < 0.05), and its pathological damage of heart, lung and kidney was serious, and the infiltration of inflammatory cells was increased(P < 0.05). Compared with sepsis model group, the levels of IFN-γ and IL-10 increased,IL-4 and IL-17 decreased, IFN-γ/IL-4 and IL-10/IL-17 increased in Shenfu injection treatment group, but were lower than those in control group, the difference were statistically significant(P < 0.05), and its pathological damage of organs was relieved and the infiltration of inflammatory cells was alleviated(all P < 0.05). Conclusion Shenfu Injection can regulate the immune system of sepsis mice, inhibit inflammation and alleviate organ damage.更多还原