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舒肝解郁胶囊对抑郁模型大鼠脑内脑源性神经营养因子的影响

Effect of Shugan Jieyu Capsules on brain-derived neurotrophic factor in model rats with depression

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【作者】 王含彦郭冬梅唐珍

【Author】 WANG Hanyan;GUO Dongmei;TANG Zhen;Teaching and Research Section of Biochemistry, School of Basic Medical Sciences, North Sichuan Medical College;

【机构】 川北医学院基础医学院生物化学教研室

【摘要】 目的探讨舒肝解郁(SGJY)胶囊对抑郁模型大鼠脑内脑源性神经营养因子(BDNF)的影响。方法将30只SD大鼠按随机数字表法随机分为五组(n=6):Control组、慢性不可预见性轻度应激(CUMS)组、氟西汀(FLU)组(FLU 2 mg/kg)、SGJY组(SGJY悬浮液150 mg/kg)、SGJY+U0126组(SGJY悬浮液150 mg/kg,0.01%U0126 5 m L/kg)。采用CUMS制作抑郁模型,各药物组连续给药21 d,Control组和CUMS组灌胃生理盐水5 m L/kg。对所有动物进行行为学测试,采用实时荧光定量PCR检测BDNF mRNA的表达,采用Western blot检测p ERK1/2蛋白表达。结果与Control组比较,其余各组用药前的穿格数、直立次数和糖水偏好均明显偏低(P <0.01)。用药后,与CUMS组比较,FLU组和SGJY组的穿格数、直立次数和糖水偏好显著增加(P <0.01或P <0.05),BDNF m RNA、p ERK1/2的表达均显著升高(P <0.01);与SGJY组比较,SGJY+U0126组穿格数、直立次数和糖水偏好显著减少(P <0.01或P <0.05),BDNF mRNA、p ERK1/2的表达均显著降低(P <0.01或P <0.05)。结论 SGJY胶囊可能通过调节ERK1/2通路增加BDNF mRNA表达从而发挥改善抑郁症状的作用。

【Abstract】 Objective To investigate the effect of Shugan Jieyu(SGJY) Capsules on brain-derived neurotrophic factor(BDNF) in model rats with depression. Methods Thirty SD rats were randomly divided into five groups(n = 6) by random number table method: control group, chronic unpredictable mild stress(CUMS) group, Fluoxetine(FLU) group(FLU2 mg/kg), SGJY group(SGJY Suspension Liquid 150 mg/kg) and SGJY+U0126 group(SGJY Suspension Liquid 150 mg/kg and 0.01% U0126 5 m L/kg). The CUMS was performed to induce depressive-like animal model. Each group was given corresponding drugs for 21 d. The control group and CUMS group were given normal saline 5 m L/kg by gavage. All animals were taken behavior tests. The expression of BDNF mRNA was detected by real-time PCR, the expression of p ERK1/2 protein was determined by Western blot. Results Compared with control group, the times of passing through grille, the upright times and sugar preference in the rest groups were all reduced before administration(P < 0.01). After administration, compared with CUMS group, the times of passing through grille, the upright times and sugar preference in FLU group and SGJY group were increased significantly(P < 0.01 or P < 0.05), the expression of BDNF mRNA,p ERK1/2 was improved significantly(P < 0.01); compared with SGJY group, the times of passing through grille, the upright times and sugar preference in SGJY+U0126 group were decreased signfiicantly(P < 0.01 or P < 0.05), the expression of BDNF mRNA, p ERK1/2 was reduced significantly(P < 0.01 or P < 0.05). Conclusion SGJY Capsules may play a role in improving the depressive symptoms by regulating the ERK1/2 pathway to increase the expression of BDNF.

【基金】 四川省教育厅重点项目(18ZA0199);四川省南充市科技局市校科技战略合作专项(18SXHZ0289)
  • 【文献出处】 中国医药导报 ,China Medical Herald , 编辑部邮箱 ,2019年20期
  • 【分类号】R285.5;R-332
  • 【被引频次】6
  • 【下载频次】138
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