【摘要】 目的观察有氧游泳运动对衰老大鼠海马中酸敏感离子通道(ASIC)表达的影响。方法选取雄性无特定病原体级SD大鼠36只,通过随机数字表法将其分为对照组、衰老模型组和衰老训练组,每组12只。衰老模型组大鼠腹腔注射D-半乳糖[150 mg/(kg·d)],衰老训练组大鼠腹腔注射D-半乳糖[150 mg/(kg·d)]同时每天有氧游泳训练60 min,共12周,对照组大鼠给予等量的生理盐水腹腔注射。Morris水迷宫实验检测大鼠的空间学习和记忆能力。Western blot检测大鼠海马中ASICs的表达。结果与对照组比较,衰老模型组大鼠逃避潜伏期的时间和到达平台游过的距离均显著延长,而大鼠穿越平台区域次数和时间明显减少,海马中ASIC1a和ASIC1b的表达显著增加,ASIC4的表达显著降低,差异有统计学意义(P <0.05)。与衰老模型组比较,衰老训练组大鼠逃避潜伏期的时间和到达平台游过的距离均显著减小,大鼠穿越平台区域次数和时间明显增加,海马中ASIC1a和ASIC1b表达显著降低,ASIC4的表达显著增加,差异有统计学意义(P <0.05)。结论有氧游泳运动改善了衰老大鼠的学习和记忆能力,其机制可能与降低海马中ASIC1a和ASIC1b表达而增加ASIC4的表达有关。更多还原

【Abstract】 Objective To observe the effects of aerobic swimming exercise on the expression of acid-sensing ion channel(ASIC) in aging rats. Methods Thirty-six male specific pathogen free(SPF) Sprague-Dawley(S-D) rats were divided into control group(C group), aging model group(AM group) and aging swimming training group(AST group) by random number table method, with 12 rats in each group. The rats in AM group were administrated with D-galactose [150 mg/(kg·d)] by intraperitoneal injection for 12 weeks. After treatment with D-galactose [150 mg/(kg·d)] by intraperitoneal injection, the rats in AST group were taken 60 minutes of aerobic swimming exercise every day for 12 weeks.The rats in C group were administrated the same amount of saline by intraperitoneal injection. The spatial learning and memory ability of rats were tested by Morris water maze test. Expression of ASIC1 a, ASIC1 b, ASIC2 a, ASIC2 b, ASIC3 and ASIC4 in hippocampus of rats were measured by Western blot. Results Compared with C group, the time of escape latency and the distance of reaching the platform were significantly increased, the number and time to across platform were significantly decreased, expression of ASIC1 a and ASIC1 b in hippocampus was significantly up-regulated and expression of ASIC4 was significantly down-regulated in AM group, the differences were statistically significant(P <0.05). Compared with the AM group, the time of escape latency and the distance to reach the platform were significantly decreased, the number and time to across platform were significantly increased, expression of ASIC1 a and ASIC1 b in hippocampus was significantly down-regulated and expression of ASIC4 was significantly up-regulated in AST group,the differences were statistically significant(P < 0.05). Conclusion The aerobic swimming exercise improves the learning and memory in aging rats, of which, the mechanism may be related with decrease of expression of ASIC1 a and ASIC1 b and increase of expression of ASIC4 in hippocampus.更多还原