【摘要】 目的:建立齿叶薰衣草药材中木犀草苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和木犀草素的含量测定方法,为其质量控制提供科学依据。方法:采用HPLC法测定;色谱条件:Phenomenex Gemini色谱柱（250 mm×4.6 mm,5μm）,流动相为乙腈和0.2%磷酸二元梯度洗脱,流速1.0 mL·min^-1,柱温30℃,检测波长350 nm。结果:木犀草苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和木犀草素的进样量在0.004～0.20、0.008～0.39、0.002～1.05、0.004～0.22μg范围内与峰面积呈良好的线性关系（r=0.9998～0.999 9）,平均回收率在101.3%%～103.6%之间（RSD<2%,n=6）。建立11批齿叶薰衣草药材HPLC指纹图谱,获得11个共有峰,从中确定了4个化学成分。结论:该方法操作简便,重复性好,可用于规范和控制齿叶薰衣草药材的质量。更多还原

【Abstract】 Objective:To establish the determination method luteoloside,luteolin-7-O-β-D-glucuronide,rosmarinic acid and luteolin in Lavandula dentata L.,so as to offer scientific basis for quality standard of the herb. Methods:The HPLC separation was performed on a Phenomenex Gemini column（250 mm×4.6 mm,5 μm）at 30 ℃ with gradient elution of acetonitrile and 0.2% phosphate acid solution at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 350 nm. Results:Luteoloside,luteolin-7-O-β-Dglucuronide,rosmarinic acid and luteolin exhibited good linearities（r=0.999 8-0.999 9）and the linear ranges were 0.004-0.20 μg,0.008-0.39 μg,0.002-1.05 μg and 0.004-0.22 μg,respectively. The average recoveries（RSD<2%,n=6）were L.. between 101.3%-103.6%. HPLC fingerprints of 11 batches of Lavandula dentata L.11 common peaks were selected and 4 of them were identified. Conclusion:The method is simple and repeatable,and it can be used for the quality control of Lavandula dentata L..更多还原