【摘要】 目的:建立同时测定补肺丸中毛蕊花糖苷（熟地黄）、东莨菪内酯（桑白皮）、五味子醇甲、五味子甲素、五味子乙素（五味子）的高效液相色谱方法和党参、黄芪、紫菀、桑白皮的薄层鉴别方法,为全面控制和提高补肺丸的质量标准提供实验依据。方法:Agilent-C₁₈色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈（A）-0.1%磷酸（B）,梯度洗脱（0～5 min,15%A→25%A;5～20 min,25%A→26%A;20～30 min,26%A→40%A;30～40 min,40%A→46%A;40～41 min,46%A→55%A;41～71 min,55%A→63%A;71～75 min,63%A）,流速1.0 mL·min^-1;柱温30℃,检测波长0～30 min（334 nm）,30～75 min（215 nm）。结果:各药材薄层色谱斑点清晰,阴性无干扰;毛蕊花糖苷、东莨菪内酯、五味子醇甲、五味子甲素和五味子乙素分别在0.020 9～0.522 5μg（r=0.999 9）,0.030 1～0.752 0μg（r=0.999 9）,0.1459～3.646 4μg（r=1.000）,0.094 3～2.358 4μg（r=1.000）,0.141 6～3.539 2μg（r=0.999 9）范围内线性关系良好,平均回收率分别为92.2%（RSD=1.6%）、92.6%（RSD=2.1%）、96.9%（RSD=2.4%）、96.8%（RSD=2.9%）和88.8%（RSD=1.3%）,6批样品中上述5个成分的含量范围分别为0.049 2～0.075 7、0.079 2～0.112 3、0.279 8～0.358 5、0.047 6～0.0693、0.146 2～0.182 1 mg·g^-1。结论:本法具有较强的专属性,准确度高,可以用于补肺丸药品质量的控制。更多还原

【Abstract】 Objective:To develop an HPLC method for the simultaneous determination of verbascoside（Rehmannia Glutinosa）,scopoletin（Mori Cortex）,schisandrin,deoxyschizandrin and γ-schisandrin（Schisandra Chinensis Fructus）and a TLC method for identification of Codonopsis Radix,Astragali Radix,Asteris Radix et Rhizoma and Mori Cortex,in order to provide experimental data for comprehensive control and improvement of Bufei pills quality standard.Methods:The separation was performed on an Agilent Welch-C₁₈（4.6 mm×250 mm,5 μm）column with acetonitrile（A）-0.1% phosphoric acid（B）as the mobile phase in the gradient elution（0-5 min,15%A → 25%A;5-20 min,25%A → 26%A;20-30 min,26%A → 40%A;30-40 min,40%A → 46%A;40-41 min,46%A → 55%A;41-71 min,55%A → 63%A;71-75 min,63%A）.The flow rate was 1.0 mL·min^-1,the detection wavelength was 334 nm（0-30 min）and 215 nm（30-75 min）,and the column temperature was maintained at 30 ℃.Results:The TLC spots of each crude drug were clear without interference from negative samples.Verbascoside,scopoletin,schisandrin,deoxyschizandrin and γ-schisandrin had good linearity within the range of 0.020 9-0.522 5 μg（r=0.999 9）,0.030 1-0.752 0 μg（r=0.999 9）,0.145 9-3.646 4 μg（r=1.000 0）,0.094 3-2.358 4 μg（r=1.000 0）and 0.141 6-3.539 2 μg（r=0.999 9）,respectively.The average recoveries were 92.2%（RSD=1.6%）,92.6%（RSD=2.1%）,96.9%（RSD=2.4%）,96.8%（RSD=2.9%）and 88.8%（RSD=1.3%）,respectively.The contents of five components in six batches of samples were 0.049 2～0.075 7 mg·g^-1 for acteoside,0.079 2～0.112 3 mg·g^-1 for scopoletin,0.279 8～0.358 5 mg·g^-1 for schisandrin,0.047 6～0.069 3 mg·g^-1 for deoxyschizandrin and 0.146 2～0.182 1 mg·g^-1 for γ-schisandrin.Conclusion:The methods standard are specific,accurate,which can be used for quality control of Bufei pills.更多还原