【摘要】 目的研究1,25-二羟基维生素D3[1,25（OH）₂D₃]对大鼠肝星状细胞系（HSC-T6）增殖、活化及细胞周期的影响,初步探讨1,25（OH）₂D₃抗纤维化的作用及机制。方法以转化生长因子-β1（TGF-β1）作为活化剂活化HSC-T6,在实验体系中加入高、中、低三个浓度的1,25（OH）₂D₃,采用MTT法测其增殖率,ELISA法测定其分泌Ⅰ型胶原蛋白（Col-Ⅰ）、Ⅲ型胶原蛋白（Col-Ⅲ）的能力,流式细胞术测定其细胞周期。结果各1,25（OH）₂D₃处理组的HSC-T6增殖能力明显低于对照组及T组（P<0.05）,随1,25（OH）₂D₃浓度升高,其对HSC-T6抑制率逐渐升高。各1,25（OH）₂D₃处理组HSC-T6培养上清Col-Ⅰ、Col-Ⅲ含量均显著低于对照组及T组（P<0.05）。各组DNA合成期（S期）细胞百分率无显著性差异（P>0.05）。VD高组、TVD中、高组DNA合成前期（G1期）细胞百分率均低于对照组及T组（P<0.05）。各1,25（OH）₂D₃处理组DNA合成后期（G2期）细胞百分率均显著高于对照组及T组（P<0.05）。结论 1,25（OH）₂D₃可抑制HSC-T6及TGF-β1活化的HSC-T6的增殖及胶原分泌,其机制可能与1,25（OH）₂D₃使HSC-T6发生G2期阻滞而抑制细胞增殖有关。更多还原

【Abstract】 Objective To study the effects of 1, 25-hydroxyvitamin D3[1, 25（OH）₂D₃] on proliferation, activation and cell cycle of rat hepatic stellate cell line（HSC-T6） in vitro, preliminary study on the anti fibrosis effect and mechanism of 1,25（OH）₂D₃. Methods HSC-T6 was activated by transforming growth factor-β1（TGF-β1） as activator, and 1,25（OH）₂D₃ with high, medium and low concentrations were added into the experimental system. The proliferation rate was measured by MTT assay, the ability to secrete type Ⅰ collagen（Col-Ⅰ） and type Ⅲ collagen（Col-Ⅲ） was determined by ELISA, the cell cycle was determined by flow cytometry. Results The proliferation ability of HSC-T6 in 1, 25（OH）₂D₃ groups were significantly lower than those in the control group and T group（P<0.05）, with the increase of 1, 25（OH）₂D₃ concentration,the inhibition rate of HSC-T6 increased gradually. The contents of Col-Ⅰ and Col-Ⅲ in the supernatant of HSC-T6 culture of 1, 25（OH）₂D₃ groups were significantly lower than those of the control group and T group（P<0.05）. There was no significant difference in the percentage of cells in DNA synthesis phase（S phase） in each group（P>0.05）. The percentage of cells in DNA pre-synthesis phase（G1 phase） in the VD high group, TVD middle and high groups were lower than those in the control group and T group（P<0.05）. The percentage of cells in DNA post-synthetic phase（G2 phase） in each 1, 25（OH）₂D₃ groups were significantly higher than those in the control group and T group（P<0.05）. Conclusion 1, 25（OH）₂D₃ can inhibit the proliferation and collagen secretion of HSC-T6 and TGF-β1 activated HSC-T6, which may be related to the G2 phase arrest of HSC-T6 caused by 1, 25（OH）₂D₃ and the inhibition of cell proliferation.更多还原