【摘要】 目的对本研究室兔胚胎-胎仔发育毒性试验中各指标的数据进行总结,建立兔胚胎-胎仔发育毒性试验指标的背景数据,为药物生殖毒性研究提供参考。方法从本研究室2011-2018年的兔胚胎-胎仔发育毒性试验中,选取全部对照组孕兔的胚胎发育和胎仔生长发育指标数据,包括孕兔的妊娠期体质量、妊娠结局（平均黄体数、平均着床数、平均活胎数、着床前丢失率、吸收胎率和死胎率）、胎仔生长发育（胎仔体质量、顶臀长和尾长）及胎仔变异率（外观、内脏、骨骼）,将数据进行统计分析,计算各指标的均数和标准差。结果统计分析了149只孕兔和1097只胎仔的胚胎-胎仔发育指标。孕兔为3～5月龄,孕兔妊娠第0天（GD₀）体质量为（3.0±0.3）kg,GD₂₈体质量为（3.5±0.4）kg。GD₂₈安乐死并剖宫检查,妊娠率为85.6%,孕兔平均黄体数为（8.0±2.1）个,平均着床数为（7.6±2.1）个,平均活胎数为（7.4±2.1）只,活胎率为96.2%,吸收胎率为1.9%,死胎率为1.8%;胎仔发育指标中,胎仔体质量为（33.4±5.0）g,胎盘重为（4.0±0.8）g,顶臀长为（86.3±5.9）mm,尾长为（11.3±0.8）mm,性别比（雄/雌）为1.0（550/547）;活胎仔中,外观变异（或畸形）率为0.18%,骨骼变异（或畸形）率为33.5%,内脏变异（或畸形）率为0.09%。结论初步建立了本研究室兔胚胎-胎仔发育毒性试验的背景数据,各指标变化比较稳定,胎仔自发畸形率较低。更多还原

【Abstract】 OBJECTIVE To summarize the data on embryo-fetal developmental toxicity study of rabbits, and to establish the background data so as to provide reference for drug reproductive toxicology research. METHODS From the embryo-fetus developmental toxicity study of rabbits in our laboratory between 2011 and 2018, all the data on control groups was selected, including rabbits′ pregnancy mass,outcomes of pregnancy（the average number of corpus lutea, implantation, live fetuses, fetal absorption rate, fetal live rate, fetal absorption rate）, fetal growth and development（mass, crown-rump length and tail length） and fetal variation（appearance, viscera and skeleton）. The mean and standard deviations of each index were calculated. RESULTS The embryo-fetal development indexes of 149 pregnant rabbits and 1097 fetuses were summarized. The gestational age of rabbits was about 3-5 months, and the body mass was（3.0±0.3） kg on gestation day 0（GD₀）, and（3.5±0.4） kg on GD₂₈. These rabbits were euthanized and cesarean section examination showed that the pregnancy rate was 85.6%, the average of corpus lutea number was 8.0±2.1, the average number of implantations was 7.6±2.1, the average number of live fetuses was 7.4±2.1, the live rate was 96.2%, the absorption rate was 1.9%, and the death rate was 1.8%. Among the fetal development indicators, fetal body mass was（33.4±5.0） g, placental weight was（4.0±0.8） g, crown-rump length was（86.3±5.9） mm, tail length was（11.3±0.8） mm, and sex ratio（male/female） was 1.0（550/547）. The rate of appearance variation（or malformation） was 0.18%, skeleton variation（or malformation） was 33.5%, and visceral variation（or malformation） was 0.09%.CONCLUSION The background data of the embryo-fetal developmental toxicity study of rabbits is established in our laboratory. The changes of each index are stable, and the spontaneous deformity rate of fetuses is low.更多还原