【摘要】 以三七的常量三醇组人参皂苷（含Re、Rg₁、R₁）为底物,研究了不同通透处理工艺对黑曲霉细胞转化人参皂苷Rh₁的影响.实验结果表明:当通透剂为3%EDTA（质量分数）,透性化处理时间为30 min （28℃）,超声处理时间为15 min时,黑曲霉细胞的通透性最佳;该条件下得到的黑曲霉细胞与三醇组人参皂苷底物反应30 min （45℃）时,Rh₁的产量达到最佳.更多还原

【Abstract】 The permeation treatment of Aspergillus niger cells producing ginsenoside Rh₁ by microbial transformation was studied, with constant protopanaxatriol of Panax notoginseng（including Re, Rg₁ and R₁） used as the substrates. The results showed that the permeability of Aspergillus niger cells was the best when the penetrating agent was 3% EDTA（mass fraction）, the permeation time was 30 minutes（28 ℃） and the ultrasonic treatment was 15 minutes. Under this condition, the Rh₁ production was optimum when the Aspergillus niger cells reacted with the protopanaxatriol for 30 minutes（45 ℃）.更多还原