【摘要】 基于硫化钼量子点（MoS₂ QDs）/过二硫酸钾（K₂S₂O₈）电化学发光（ECL）体系,构建了灵敏检测心肌肌钙蛋白Ⅰ（cTnⅠ）无标记型免疫传感器新方法.将发光材料MoS₂ QDs与还原氧化石墨烯（rGO）复合形成MoS₂ QDs-rGO纳米复合物,然后组装构建传感器.在优化条件下,对cTnI检测的线性范围为1.0×10^-9～1.0×10^-4 g/L（R=0.998）,检出限为4.9×10^-10 g/L(R_SN=3).将提出的方法应用于实际人血清样品中cTnⅠ含量的测定,RSD在1.0%～2.6%范围,加标回收率在98.0%～110.0%之间.讨论了反应机理.更多还原

【Abstract】 A label-free electrochemiluminescence（ECL）immunosensor for sensitive detection of cardiac troponin Ⅰ（cTnⅠ）was constructed based on MoS₂ quantum dots（MoS₂ QDs）/potassium persulfate（K₂S₂O₈）ECL system.The luminescent material MoS₂ QDs was combined with reduced graphene oxide（MoS₂ QDs-rGO）to construct the immunosensor.The large surface area of rGO could carry a large amount of MoS₂ QDs.The MoS₂ QDs-rGO exhibited good ECL performance.Under the optimized conditions,the proposed ECL immunosensing method showed good analytical performance for cTnI detection,and the linear range was 1.0×10^-9～1.0×10^-4 g/L（R = 0.998）with a detection limit of 4.9×10^-10 g/L(R_SN=3).The proposed method was applied for the determination of cTnI in human serum samples,and the analytical results were consistent with the results of Xinyang Central Hospital.The recoveries of the method were 98.0%～110.0%.更多还原