【摘要】 目的探讨白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)基因多态性与急性脑梗死的相关性。方法选择2016年4月至2017年8月新乡医学院第二附属医院神经内科收治的86例脑梗死患者为研究对象(脑梗死组),另选择同期健康体检者92例为对照组。采用序列特异性引物和聚合酶链式反应(PCR)、限制性内切酶酶切技术检测2组受试者血液中IL-1β(-511) C/T、TNF-α(-238) G/A基因多态性。结果 2组受试者IL-1β(-511)位点基因型频率和TNF-α(-238)位点基因型频率均符合Hardy-Weinberg遗传平衡定律(P>0. 05)。脑梗死组患者IL-1β(-511)位点TT基因型频率高于对照组(OR=2.059,95%可信区间:1.087～3.899,P <0.05);脑梗死组患者IL-1β(-511)位点C等位基因频率低于对照组(OR=0.486,95%可信区间:0.256～0.920,P <0.05)。2组受试者TNF-α(-238)位点基因型频率和等位基因频率比较差异无统计学意义(P>0.05)。其中AA基因型在全部研究对象中未检出,G等位基因见于全部研究对象。结论 IL-1β(-511) C/T多态性可能与脑梗死有关,TNF-α(-238) G/A多态性可能与脑梗死无关。更多还原

【Abstract】 Objective To investigate the relationship between interleukin-1β( IL-1β),tumor necrosis factor-α( TNF-α)gene polymorphism and acute cerebral infarction. Methods Eighty-six patients with cerebral infarction hospitalized in the Department of Neurology of the Second Affiliated Hospital of Xinxiang Medical University from April 2016 to August 2017 were selected as the study objects( cerebral infarction group),and 92 healthy people with physical examination during the same period were selected as the control group. The polymorphisms of IL-1β(-511) C/T and TNF-α(-238) G/A genes in the blood of the subjects in the two groups were detected by sequence specific primers,polymerase chain reaction( PCR) and restriction enzyme digestion. Results The genotype frequency of IL-1β(-511) locus and TNF-α(-238) locus in the two groups were consistent with Hardy-Weinberg genetic equilibrium law( P > 0. 05). The TT genotype frequency of IL-1β(-511) in the cerebral infarction group was higher than that in the control group( OR = 2. 059,95% CI: 1. 087-3. 899,P < 0. 05). The C allele frequency of IL-1β(-511) in the cerebral infarction group was lower than that in the control group( OR = 0. 486,95% CI:0. 256-0. 920,P < 0. 05). There was no significant difference in the genotype frequency and allele frequency of TNF-α(-238)locus between the two groups( P > 0. 05). Among them,AA genotype of TNF-α(-238) locus was not detected in all subjects,and G allele of TNF-α(-238) locus was found in all subjects. Conclusion IL-1β(-511) C/T polymorphism may be associated with cerebral infarction,and TNF-α(-238) G/A polymorphism may not be associated with cerebral infarction.更多还原