【摘要】 目的探讨河南省精神疾病患者弓形虫感染情况。方法采集2013年5月至2014年12月新乡医学院第二附属医院收治的156例精神疾病患者的血液样本（病例组）和新乡医学院第三附属医院收治的150例非精神疾病患者血液样本（对照组）,采用抗弓形虫IgG抗体酶联免疫吸附试验试剂盒检测2组患者血清弓形虫特异性Ig G抗体,采用聚合酶链反应（PCR）检测2组患者外周血白细胞DNA标本中弓形虫特异性529片段,采用荧光定量PCR技术检测2组患者血液中弓形虫虫体含量。比较病例组不同年龄、性别、疾病类型患者弓形虫感染情况。结果对照组患者既往感染率、现症感染率及持续慢性感染率比较差异均无统计学意义（P> 0. 05）;病例组患者既往感染率高于现症感染率和持续慢性感染率（P <0. 01）,现症感染率与持续慢性感染率比较差异无统计学意义（P> 0. 05）。病例组患者弓形虫感染总阳性率显著高于对照组（P <0. 01）。病例组和对照组患者血细胞中弓形虫虫体拷贝数分别为（4. 057±1. 979）×10⁷、（1. 089±0. 014）×106copies·L^-1;病例组患者血细胞中弓形虫虫体拷贝数高于对照组（P <0. 01）。病例组各年龄段患者弓形虫既往感染率、持续慢性感染率及感染总阳性率比较差异均无统计学意义（P>0. 05）; 40～49岁组患者现症感染率高于10～19岁、20～29岁、30～39岁及> 50岁组患者（P <0. 05）,10～19岁、20～29岁、30～39岁及>50岁组患者的现症感染率比较差异均无统计学意义（P> 0. 05）。10～19岁、20～29岁、30～39岁、>50岁组患者既往感染率高于现症感染率、持续慢性感染率（P <0. 01）,现症感染率与持续慢性感染率比较差异均无统计学意义（P>0. 05）。40～49岁组患者的既往感染率高于持续慢性感染率（P <0. 05）;现症感染率与既往感染率、持续慢性感染率比较差异均无统计学意义（P>0. 05）。不同性别患者之间的既往感染率、现症感染率、持续慢性感染率及感染总阳性率比较差异均无统计学意义（P> 0. 05）。男性和女性患者的既往感染率均高于现症感染率、持续慢性感染率（P <0. 01）,现症感染率与持续慢性感染率比较差异均无统计学意义（P>0. 05）。精神分裂症患者与其他精神疾病患者既往感染率、现症感染率、持续慢性感染率及感染总阳性率比较差异均无统计学意义（P> 0. 05）。精神分裂症患者和其他精神疾病患者的既往感染率均高于现症感染率、持续慢性感染率（P <0. 01）,现症感染率与持续慢性感染率比较差异均无统计学意义（P>0. 05）。结论精神疾病患者弓形虫感染率高于非精神疾病患者,且不同年龄、性别及疾病类型精神疾病患者的既往感染率均高于现症感染率和持续慢性感染率,弓形虫感染可能是精神疾病发病原因之一。更多还原

【Abstract】 Objective To study the infection of Toxoplasma gondii in patients with mental illness in Henan province.Methods Blood samples were collected from 156 patients with mental illness( case group) admitted to the Second Affiliated Hospital of Xinxiang Medical University and 150 patients without mental illness( control group) admitted to the Third Affiliated Hospital of Xinxiang Medical University from May 2013 to December 2014. The serum Toxoplasmo-specific IgG antibody of patients in the two groups was detected by anti-toxoplasma gondii Ig G antibody elisa kit,the Toxoplasmo-specific529 fragments in the DNA samples of peripheral blood leukocytes of patients in the two groups was detected by polymerase chain reaction( PCR) and the parasite content in the blood of patients in the two groups was detected by quantitative fluorescence PCR. The Toxoplasma infection was compared in patients with different age,sex and disease type in case group.Results There was no significant difference in the previous infection,current infection and persistent chronic infection rate of patients in the control group( P > 0. 05). The previous infection rate was higher than the current infection rate and the persistent chronic infection rate of patients in the case group( P < 0. 01); the difference between the present infection rate and the persistent chronic infection rate was not significant of patients in the case group( P > 0. 05). The total infection rate of Toxoplasma gondii in the case group was significantly higher than that in the control group( P < 0. 01). The copies of Toxoplasma gondii in the blood of patients in the case group and control group were( 4. 057 ± 1. 979) × 107,( 1. 089 ±0. 014) × 106 copies·L-1; the copies of parasite of patients in the case group was higher than that in the control group( t =0. 580,P < 0. 01). There was no significant difference in the previous infection rate,persistent chronic infection rate and total positive rate among different age patients in case group( P > 0. 05); the current infection rate of 40-49 years old group was higher than that of 10-19,20-29,30-39 and > 50 years old groups( P < 0. 05),and there was no significant difference in the current infection rate among 10-19,20-29,30-39 and > 50 years old age group( P > 0. 05). The previous infection rate was higher than the present infection rate and the persistent chronic infection rate in the 10-19,20-29,30-39 and > 50 years old group( P < 0. 01); but there was no statistic difference between the current infection rate and chronic infection rate in the 10-19,20-29,30-39 and > 50 years old group( P > 0. 05). The previous infection rate was higher than the persistent chronic infection rate in 40-49 years old group( P < 0. 05); there was no significant difference between the present infection rate and the previous infection rate,the persistent chronic infection rate in 40-49 years old group( P > 0. 05). There was no statistic difference in the previous infection rate,present infection rate,persistent chronic infection rate and the total positive rate between male and female patients( P > 0. 05). The previous infection rate was higher than the current infection rate and the persistent chronic infection rate in both male and female patients( P < 0. 01),but there was no statistic difference between the current infection rate and the persistent chronic infection rate in male and female patients( P > 0. 05). There was no statistic difference in the previous infection rate,present infection rate,persistent chronic infection rate and the total positive rate between chizophrenia patients and other mental diseases patients( P > 0. 05). The previous infection rate was higher than the current infection rate and the persistent chronic infection rate in both chizophrenia patients and other mental diseases patients( P < 0. 01),but there was no statistic difference between the current infection rate and the persistent chronic infection rate in chizophrenia patients and other mental diseases patients( P > 0. 05). Conclusion The infection rate of Toxoplasma gondii in mental illness patients is higher than non mental illness patients,and the previous infection rate is higher than the current infection rate and the persistent chronic infection rate in mental illness patients with different age,gender,type of disease; which suggest that Toxoplasma gondii infection may be one cause of mental illness.更多还原