【摘要】 目的探讨B族Ⅰ型清道夫受体（SR-BⅠ）在人冠状动脉平滑肌细胞（hCASMC）增殖和迁移过程中的作用及机制。方法常规培养hCASMC,根据处理方法将细胞分为空白对照组、5μg·L^-1血小板源性生长因子BB（PDGF-BB）组、10μg·L^-1PDGF-BB组、20μg·L^-1PDGF-BB组、20μg·L^-1PDGF-BB+腺病毒绿色荧光蛋白（AdGFP）组（PDGF-BB+Ad-GFP组）、20μg·L^-1PDGF-BB+Ad-GFP-SR-BⅠ组（PDGF-BB+Ad-GFP-SR-BⅠ组）。采用噻唑兰法检测各组细胞增殖情况,Western blot法检测各组细胞中SR-BⅠ表达,Transwell法检测各组细胞迁移情况。结果空白对照组及5、10、20μg·L^-1PDGF-BB组细胞中SR-BⅠ相对表达量分别为1.00±0.05、0.76±0.08、0.52±0.06、0.30±0.05,5、10、20μg·L^-1PDGF-BB组细胞SR-BⅠ相对表达量均显著低于空白对照组（P<0.05）,10、20μg·L^-1PDGF-BB组细胞SR-BⅠ相对表达量均显著低于5μg·L^-1PDGF-BB组（P<0.05）,20μg·L^-1PDGF-BB组细胞SR-BⅠ相对表达量显著低于10μg·L^-1PDGF-BB组（P<0.05）。培养24、48、72 h时,5、10、20μg·L^-1PDGF-BB组及PDGF-BB+Ad-GFP组、PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力均显著高于空白对照组（P<0.05）,10、20μg·L^-1PDGF-BB组及PDGF-BB+Ad-GFP组、PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力显著高于5μg·L^-1PDGF-BB组（P<0.05）,20μg·L^-1PDGF-BB组、PDGF-BB+Ad-GFP组、PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力显著高于10μg·L^-1PDGF-BB组（P<0.05）; PDGF-BB+Ad-GFP-SR-BⅠ组细胞增殖能力低于20μg·L^-1PDGF-BB组和PDGF-BB+Ad-GFP组（P<0.05）; 20μg·L^-1PDGF-BB组与PDGF-BB+Ad-GFP组细胞增殖能力比较差异无统计学意义（P>0.05）。空白对照组、20μg·L^-1PDGF-BB组、PDGF-BB+Ad-GFP组及PDGFBB+Ad-GFP-SR-BⅠ组细胞迁移数分别为24.2±3.6、76.6±4.2、75.2±4.8和60.6±3.6,各组细胞迁移数比较差异有统计学意义（F=40.695,P<0.01）; 20μg·L^-1PDGF-BB组、PDGF-BB+Ad-GFP组及PDGF-BB+Ad-GFP-SR-BⅠ组细胞迁移数显著高于空白对照组,PDGF-BB+Ad-GFP-SR-BⅠ组细胞迁移数显著低于20μg·L^-1PDGF-BB组、PDGF-BB+Ad-GFP组（P<0.05）; 20μg·L^-1PDGF-BB组与PDGF-BB+Ad-GFP组细胞迁移数比较差异无统计学意义（P>0.05）。结论 PDGF-BB能够促进h CASMC增殖和迁移,并减少SR-BⅠ表达,过表达SR-BⅠ能够抑制PDGFBB的作用,抑制h CASMC增殖和迁移。更多还原

【Abstract】 Objective To investigate the role and mechanism of scavenger receptor class B typeⅠ（SR-BⅠ） in the proliferation and migration of human coronary artery smooth muscle cells（h CASMC）. Methods h CASMC were routinely cultured and divided into blank control group,5 μg·L^-1 platelet-derived growth factor BB（PDGF-BB） group,10 μg·L^-1 PDGF-BB group,20 μg·L^-1 PDGF-BB group,20 μg·L^-1 PDGF-BB + adenovirus green fluorescent protein（Ad-GFP）group（PDGF-BB + Ad-GFP group） and 20 μg·L^-1 PDGF-BB + Ad-GFP-SR-BⅠ group according to the treatment method.The cell proliferation was detected by thiazolam method,SR-B Ⅰ expression was detected by Western blot and the cell migration was detected by Transwell method. Results The relative expression of SR-BⅠ in the blank control group and 5,10,20 μg·L^-1 PDGF-BB group was 1.00 ± 0.05,0.76 ± 0.08,0.52 ± 0.06 and 0.30 ± 0.05,respectively. There was significant difference in the relative expression of SR-BⅠ in the each group. The relative expression of SR-BⅠ in the 5,10,20 μg·L^-1 PDGF-BB group was significantly lower than that in the blank control group（P<0.05）. The relative expression of SR-BⅠ in the 10,20 μg·L^-1 PDGF-BB group was significantly lower than that in the 5 μg·L^-1 PDGF-BB group（P<0.05）,and the relative expression of SR-BⅠ in the 20 μg·L^-1 PDGF-BB group was significantly lower than that in the 10 μg·L^-1 PDGFBB group（P<0.05）. After culturing for 24,48,72 h,the proliferative ability in the 5,10,20 μg· L-1 PDGF-BB group,PDGF-BB + Ad-GFP group and PDGF-BB + Ad-GFP-SR-B Ⅰ group was significantly higher than that in the blank control group（P<0.05）; the proliferative ability in the 10,20 μg·L^-1 PDGF-BB group was significantly higher than that in the5 μg·L^-1 PDGF-BB group（P<0.05）; the proliferative ability in the 20 μg·L^-1 PDGF-BB group was significantly higher than that in the 10 μg·L^-1 PDGF-BB group（P<0.05）; the proliferative ability in the PDGF-BB + Ad-GFP-SR-BⅠ group was lower than that in the 20 μg·L^-1 PDGF-BB group and PDGF-BB + Ad-GFP group（P<0.01）; but there was no significant difference between the 20 μg·L^-1 PDGF-BB group and PDGF-BB + Ad-GFP group（P>0.05）. The number of migrating cells in the blank control group,20 μg·L^-1 PDGF-BB group,PDGF-BB + Ad-GFP group and PDGF-BB + Ad-GFPSR-BⅠ group was 24.2 ± 3.6,76.6 ± 4.2,75.2 ± 4.8 and 60.6 ± 3.6; there was significant difference in the number of migrating cells among the groups（F=40.695,P<0.01）; the number of migrating cells in the 20 μg·L^-1 PDGF-BB group,PDGF-BB + Ad-GFP group and PDGF-BB + Ad-GFP-SR-B Ⅰ group was significantly higher than that in the blank control group; and the number of migrating cells in the PDGF-BB + Ad-GFP-SR-BⅠ group was lower than that in the 20 μg·L^-1 PDGF-BB group and PDGF-BB + Ad-GFP group（P<0.05）; but there was no significant difference between the 20 μg·L^-1 PDGF-BB group and PDGF-BB + Ad-GFP group（P>0.05）. Conclusion PDGF-BB can promote the proliferation and migration of h CASMC,and reduce the expression of SR-BⅠ. SR-BⅠ overexpression can inhibit the effect of PDGF-BB and the proliferation and migration of PDGF-BB and h CASMC cells.更多还原