【摘要】 目的初步探讨丙泊酚抑制过氧化氢诱导细粒棘球蚴原头节凋亡作用机制。方法应用体外培养技术,通过伊红染色法定时在光学显微镜下观察各组原头节的活力并记录存活和死亡数目。使用试剂盒及DCFH-DA染色法荧光显微镜观察检测0.5 mmol/L H₂O₂组及1 mmol/L丙泊酚+0.5 mmol/L H₂O₂组原头节体内HO-1酶活性及ROS水平的变化。结果 DMSO及不同浓度的丙泊酚体外作用于原头节24 h,原头节的活力均大于95.0%。0.5 mmol/L H₂O₂作用于原头节6 h,原头节活力为38.0%。丙泊酚（0.5 mmol/L、0.75 mmol/L、1 mmol/L）预处理8 h后加入0.5 mmol/L H₂O₂共培养6 h,原头节的存活率明显增加（P<0.05）。1 mmol/L丙泊酚预处理原头节8 h后加入0.5 mmol/L H₂O₂共培养6 h,HO-1酶活性较单独使用0.5 mmol/L H₂O₂组明显增加,ROS水平相比较则相反。结论丙泊酚对原头节无毒性且具有抑制过氧化氢诱导细粒棘球蚴原头节凋亡作用,其机制可能与增加HO-1酶活性及降低原头节内ROS水平有关,具体机制有待进一步研究。更多还原

【Abstract】 Objective To investigate the mechanism of propofol inhibition of hydrogen peroxide-induced apoptosis in the protoplast of Echinococcus granulosus. Methods The vitro culture technique was used to observe the viability of the original head sections of each group under the light microscope and record the number of survival and death by eosin staining. The HO-I enzyme activity and ROS level in the original ganglion of 0.5 mmol/L H₂O₂ group and 1 mmol/L propofol + 0.5 mmol/L H₂O₂ group were detected by fluorescence microscopy and DCFH-DA staining. Results DMSO and different concentrations of propofol acted on the original nodule 24 h in vitro, and the viability of the original head section was greater than 95.0%. 0.5 mmol/L H₂O₂ was applied to the original head section for 6 h, and the original head section activity was 38.0%. Propofol（0.5 mmol/L, 0.75 mmol/L, 1 mmol/L） was pretreated for 8 h and then co-cultured with 0.5 mmol/L H₂O₂ for 6 h. The survival rate of the original nodule was significantly increased（P <0.05）. After 1 mmol/L propofol was pretreated for 8 h, 0.5 mmol/L H₂O₂ was added for 6 h. The activity of HO-1 was significantly increased compared with 0.5 mmol/L H₂O₂ alone, and the ROS level was reversed. Conclusion Propofol is non-toxic to the protoplast and has the effect of inhibiting the apoptosis of the protoplast of the echinococcosis by hydrogen peroxide. The mechanism may be related to the increase of HO-1 enzyme activity and the reduction of ROS level in the original nodule. The mechanism needs further study.更多还原