【摘要】 【目的】RACK1可与多种信号分子相互作用并影响不同的信号转导途径,可能参与了植物在生物和非生物逆境胁迫中的多种应答。【方法】为研究羽衣甘蓝RACK1对抗病性中的功能,通过农杆菌介导的遗传转化方法获得转化植株,并对3个独立转基因株系进行抗逆性分析。【结果】接种后转基因植株表现明显的抗病表型,抗病防御相关基因和表达量显著高于野生型。【结论】RACK1基因在羽衣甘蓝抗病过程中具有正调控作用,通过上调逆性相关基因的表达量增强抗逆性。更多还原

【Abstract】 【Objective】RACK1 can interact with a variety of signal molecules and affect different signal transduction pathways, which involves in various responses of plants to biotic and abiotic stress. 【Method】In order to study the function of RACK1 resistance to disease, a transgenic plant by Agrobacterium mediated transformation was obtained, and 3 independent transgenic lines were analyzed for resistance. 【Result】The transgenic plants displayed obvious disease resistance after inoculation, and the expression levels of the resistance related genes were significantly higher than that of the wild type. 【Conclusion】These results indicated that the RACK1 gene played a positive role in the process of disease resistance in Brassica oleracea and increased the resistance by up regulating the expression of the related genes.更多还原