【摘要】 旨在揭示牦牛泌乳中期和静止期乳腺组织雌激素受体α(ERα)和雌激素受体β(ERβ)的分布及蛋白水平的表达。采集泌乳中期(分娩后3～4个月)和静止期(断乳后1个月)各5头健康牦牛的乳腺组织,应用SP免疫组织化学染色方法和Image pro plus 6.0图像分析软件检测ERα和ERβ在牦牛泌乳中期和静止期的分布情况,并采用Western blot技术对乳腺组织内的ERα和ERβ蛋白含量进行半定量测定。结果表明:泌乳中期牦牛乳腺组织内ERα大多分布于腺泡上皮细胞的细胞质、少部分分布于小叶间结缔组织内脂肪细胞的细胞核、偶见于血管内皮细胞的细胞核;静止期牦牛乳腺组织内ERα大量分布于结缔组织中脂肪细胞及血管内皮细胞的细胞核中,少数分布于腺泡上皮细胞的细胞质中;泌乳中期ERβ大多分布于乳腺腺泡上皮细胞的细胞质,偶见于血管内皮细胞及脂肪细胞的细胞核中,但着色较腺泡上皮细胞的细胞质浅。静止期ERβ广泛分布于乳腺导管上皮细胞的细胞质、小叶间结缔组织内间质细胞的细胞核及血管内皮细胞的细胞核中,腺泡上皮细胞的细胞质内也有表达,但较泌乳中期阳性细胞数少。牦牛乳腺组织内ERα蛋白的相对表达量静止期显著高于泌乳中期(P<0.05);静止期与泌乳中期乳腺组织内ERβ蛋白的相对表达量无显著差异(P>0.05)。雌激素受体不同亚型在不同发育期牦牛乳腺组织内的表达存在差异,提示,雌激素受体不同亚型在牦牛乳腺发育及泌乳生物学中可能执行不同的功能。更多还原

【Abstract】 In order to reveal the distribution and expression of estrogen receptorα (ERα) and estrogen receptorβ (ERβ) in mammary gland of yak during mid-lactating and non-lactating periods.The mammary tissues from each of 5healthy yaks in the mid-lactating (3-4months after parturition) and non-lactating periods (1month after weaning) were collected,the localization,distribution and protein content of ERαand ERβwere tested by using the SP immunohistochemical staining method and the Western blot method,respectively.The results showed that predominantcytoplasmic staining for ERαwere found in gland bubble epithelial cells and weak nuclear staining for ERαwere found in fat cells in interlobular connective tissue and vascular endothelial cells in mammary gland during mid-lactating period.But during non-lactating period,distribution of ERα-positive material was contrary to that in mid-lactating period.Predominant cytoplasmic staining for ERβwere found in the gland bubble epithelial cells during mid-lactating period and weak nucleus staining for ERβwere found in vascular endothelial cells and fat cells.During non-lactating period,ERβ-positive material were found in cytoplasm of duct epithelial cells,in the nucleus of stroma cells in interlobular connective tissue and vascular endothelial cells,and in cytoplasm of acinar epithelial cells,but the number of positive cells was less than that during mid-lactating period.The relative expression of ERαprotein in yak mammary gland tissue in non-lactating period was significantly higher than that in mid-lactating period (P<0.05),while there was no significant difference in the relative expression of ERβprotein between mid-lactating and non-lactating periods (P>0.05).The different expression patterns of estrogen receptor subtypes in mammary gland of yak in different periods suggested that ERαand ERβin mammary gland of yak might perform different functions during the process of mammary development and lactation.更多还原