【摘要】 为深入研究枣体细胞胚胎发生的形态建成过程,以‘小梨枣’幼胚为材料,采用苏木精-伊红法染色和石蜡切片技术对体细胞胚发生、发育进行了组织学观察。结果表明:胚型愈伤组织细胞体积小,细胞核大,细胞质浓,细胞排列紧密;而非胚性愈伤组织细胞体积大,细胞核小,细胞质稀薄。体细胞胚产生于幼胚愈伤组织的表层细胞或内部细胞,且发生不同步,其发育过程与合子胚发育过程相似,即经历球形期、心形期和鱼雷期,最终形成子叶形胚。更多还原

【Abstract】 The paper aims to study the morphogenesis process of somatic embryogenesis. We used young embryos of Ziziphus jujuba‘Xiaolizao’as explants, and carried out the histological observation of somatic embryo formation and development by using the method of hematoxylin-eosin staining and paraffin section.The results indicated that: the embryogenic callus cells had the characteristics of small size, large nucleus,thick cytoplasm and the cells were closely arranged; however, the non-embryogenic callus cells were characterized with large size, small nucleus and thin cytoplasm. The somatic embryos were originated from surface calluscells and inner calluscells of immature embryos and their development were not synchronized which was similar to the embryonic development process of zygotes, the somatic embryos also experienced the spherical stage, the heart-shaped stage, the torpedo stage and ultimately formed cotyledon embryos.更多还原