【摘要】 目的探讨环状RNA（circRNA）hsa_circ₀011021在宫颈癌组织和细胞株中的表达变化及其对宫颈癌细胞增殖、迁移和侵袭能力的影响。方法选取河南科技大学第一附属医院就诊的15例宫颈癌患者,取15对宫颈癌组织和癌旁组织。qRT-PCR实验检测hsa_circ₀011021在宫颈癌组织和宫颈癌癌旁组织（Hela和SiHa）细胞中的相对表达水平;CCK-8和克隆形成实验检测沉默hsa_circ₀011021对Hela细胞活性和克隆形成能力的影响;划痕愈合实验和Transwell侵袭实验检测沉默hsa_circ₀011021对Hela细胞迁移及侵袭能力的影响。结果 hsa_circ₀011021在宫颈癌组织的相对表达水平明显高于癌旁组织（P<0.05）;hsa_circ₀011021在Hela和SiHa细胞中的相对表达水平明显高于人永生化表皮细胞HaCaT（P<0.05）。在Hela细胞沉默hsa_circ₀011021,Hela细胞的活性和克隆形成能力显著降低,迁移距离明显减少,侵袭能力也明显下降（P<0.05）。结论 hsa_circ₀011021在宫颈癌组织和宫颈癌细胞（Hela和SiHa）中的表达水平明显升高;沉默hsa_circ₀011021显著抑制宫颈癌Hela细胞的增殖、迁移和侵袭。更多还原

【Abstract】 Objective To explore the expression of the circular RNA（circRNA） hsa_circ₀011021 in cervical cancer tissues and cell lines, and study the effect of hsa_circ₀011021 knockdown on the proliferation, migration and invasion of cervical cancer cells. Methods The expression levels of hsa_circ₀011021 in cervical cancer tissues and cervical cancer cell lines（Hela and SiHa） were determined by quantitative real-time PCR（qRT-PCR）. The effect of hsa_circ₀011021 on the viability and proliferation of cervical cancer cells, CCK-8 and colony formation assays were performed in Hela cells were observed. Transwell migration assay and wound scratch assay were carried out to evaluate the effect of hsa_circ₀011021 on the migration and invasion ability of Hela cells.Results The expression level of hsa_circ₀011021 in cervical cancer tissues and Hela and SiHa cells was higher than that in adjacent normal tissues and human immortal keratinocyte line（HaCaT）. Knockdown of hsa_circ₀011021 obviously inhibited the proliferation, migration and invasion of Hela cells. Conclusion Knockdown of hsa_circ₀011021 remarkably suppresses the proliferation, migration and invasion of cervical cancer cells.更多还原