【摘要】 背景:前期实验构建了羟基磷灰石/β-磷酸三钙/壳聚糖/甲基纤维素三维复合支架材料,发现其具有良好的理化性能和生物相容性。目的:评估可注射性羟基磷灰石/β-磷酸三钙/壳聚糖/甲基纤维素支架复合骨髓间充质干细胞用于兔上颌窦提升的成骨效果。方法:取24只新西兰大白兔(新疆医科大学动物实验中心提供),每只兔均制作4处上颌窦提升区域,其中3处分别植入可注射性羟基磷灰石/β-磷酸三钙/壳聚糖/甲基纤维素支架与骨髓间充质干细胞复合物(实验组)、可注射性羟基磷灰石/β-磷酸三钙/壳聚糖/甲基纤维素支架(对照组1)、羟基磷灰石/β-磷酸三钙骨粉(对照组2),最后一处不植入任何材料,作为空白对照。术后4,8,12周,取上颌窦提升区域标本,分别进行锥形束CT扫描、苏木精-伊红染色与免疫组织学化染色观察。实验经新疆医科大学第一附属医院实验动物伦理委员会审议批准(批准编号IACUC20170706-02)。结果与结论:①锥形束CT显示,空白对照组始终无新骨生成,其余3组术后8周时可见新骨生成,且以实验组成骨效果最明显;术后12周时,实验组、对照组1、对照组2成骨效果进一步增强,3组间差异不明显;②苏木精-伊红染色显示,实验组术后8,12周的成骨效果优于对照组1、对照组2(P <0.05);③免疫组织化学染色显示,实验组术后8周的成骨指标骨形态发生蛋白2、血管内皮生长因子表达均强于对照组1、对照组2(P <0.05);术后12周时,3组间两指标表达差异不明显(P> 0.05);④结果表明,羟基磷灰石/β-磷酸三钙/壳聚糖/甲基纤维素可注射组织工程骨可促进上颌窦提升区域的骨形成。更多还原

【Abstract】 BACKGROUND: Three-dimensional scaffold material hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose has been shown to exhibit good physical and chemical properties and biocompatibility.OBJECTIVE: To evaluate the osteogenic effects of injectable hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffold material compounded with bone marrow mesenchymal stem cells used for maxillary sinus elevation in rabbits.METHODS: Twenty-four New Zealand rabbits were provided by Laboratory Animal Center of Xinjiang Medical University, China and included in this study. Four maxillary sinus elevation areas were created in each rabbit. Injectable hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffold compounded with bone marrow mesenchymal stem cell complex(experimental group), simple injectable hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffold(control group 1), or hydroxyapatite/β-tricalcium phosphate bone powder(control group 2) were implanted into three of the four maxillary sinus elevation areas. For blank control, no material was implanted in the last area. At 4, 8 and 12 weeks after surgery, maxillary sinus elevation area specimens were taken for cone-beam CT scanning,hematoxylin-eosin staining, and immunohistochemical staining. The experiments were approved by the Laboratory Animal Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University(approval No. IACUC201706-02).RESULTS AND CONCLUSION:(1) Cone beam CT scanning showed that there was no newly formed bone in the blank control group, and new bone formed in the other three groups, in particular in the experimental group, at 8 weeks after surgery. A greater amount of bone formed in the experimental group, control group 1, and control group 2 at 12 weeks after surgery compared with the blank control group. There was no significant difference among three groups.(2) Hematoxylin-eosin staining showed that at 8 and 12 weeks after surgery, the osteogenic effect in the experimental group was superior to that in the control groups 1 and 2(P < 0.05).(3) Immunohistochemical staining showed that at8 weeks after surgery, the expression level of osteogenic markers bone morphogenetic protein 2 and vascular endothelial growth factor in the experimental group was higher than that in the control groups 1 and 2(P < 0.05). At 12 weeks after surgery, there were no significant differences in the expression levels of bone morphogenetic protein 2 and vascular endothelial growth factor among three groups(P > 0.05).(4) These results suggest that injectable hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffolds can promote the bone formation in the maxillary sinus elevation areas.更多还原