【摘要】 背景:糖代谢紊乱是糖尿病的主要临床表现,运动是治疗糖尿病的主要方法之一,早期不同负荷运动对后期2型糖尿病大鼠模型成模率及血糖水平的影响尚不明确。目的:探究不同负荷运动对高糖高脂膳食联合注射链脲佐菌素建立大鼠2型糖尿病模型的影响。方法:50只SD雄性大鼠(购自湖北省实验动物研究中心),随机分为安静对照组、糖尿病对照组、小强度运动干预组、中等强度运动干预组和大强度运动干预组5组,每组10只。安静对照组普通饲料喂养;糖尿病对照组高糖高脂饲料喂养,均笼中自由活动;小、中、大强度运动干预组高糖高脂喂养,期间分别进行8周不同强度的跑台运动。运动第8周末,检测各组大鼠的体质量和空腹血糖,然后隔夜禁食不禁水12 h,安静对照组一次性腹腔注射柠檬酸缓冲液,其余4组一次性腹腔注射2%链脲佐菌素溶液。注射链脲佐菌素第7天再次检测大鼠体质量、空腹血糖和糖化血清蛋白水平。结果与结论:不同强度运动干预组的体质量、空腹血糖、糖化血清蛋白、血糖曲线下面积均较糖尿病对照组有所改善,中等、大强度运动干预可更有效地调节2型糖尿病血糖水平,改善糖耐量,而大强度运动干预对于降低2型糖尿病的发病率更为有效。更多还原

【Abstract】 BACKGROUND: Dysfunction of glucose metabolism is the main clinical manifestation of diabetes mellitus.Exercise is one of the main methods to treat diabetes mellitus. The effect of early exercise with different loads on the modeling rate and blood glucose level of late-stage type 2 diabetic rats is unclear. OBJECTIVE: To explore the effects of different exercise loads on the establishment of rat diabetes mellitus model with high-sugar and high-fat diet combined with streptozotocin injection. METHODS: Fifty Sprague-Dawley male rats(provided by Hubei Provincial Laboratory Animal Research Center) were randomly divided into quiet, diabetes mellitus, and low-, moderate-, and high-intensity exercise groups(n=10/group). Rats in the quiet group were fed with normal feed, diabetes mellitus group were fed with high-sugar and high-fat diets, and were freely active in cages. Mice in the low-, moderate-, and high-intensity exercise groups were fed with high-sugar and high-fat diets, and underwent 8-week exercise of different intensities. At the end of exercise, the body weight and free blood glucose in each group were tested, and then the rats were fasted but free to drink for 12 hours overnight. Quiet group was given intraperitoneal injection of citrate buffer, and other groups were injected intraperitoneally with 2% streptozotocin solution. The body weight, free blood glucose and glycated serum protein on day 7 were detected. RESULTS AND CONCLUSION: The body weight, free blood glucose, glycated serum protein, and area under the curve of blood glucose in each exercise group were improved compared with the diabetes mellitus group. Moderate-and high-intensity exercise can effectively regulate blood glucose level and improve glucose tolerance of type 2 diabetes mellitus. Besides, high-intensity exercise is more effective in reducing the incidence of type 2 diabetes mellitus.更多还原