节点文献

山药粘液质多糖的酶法脱蛋白工艺及其性能研究

Deproteinization Technology by Enzymic Method of Polysaccharide from Yam Mucilage and Its Performance Study

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 宁奇孙培冬曹光群张晨邬凤娟

【Author】 NING Qi;SUN Peidong;CAO Guangqun;ZHANG Chen;WU Fenjuan;School of Chemical and Material Engineering ,Jiangnan University;Infinitus (China)Company Ltd.;

【通讯作者】 孙培冬;

【机构】 江南大学化学与材料工程学院无限极(中国)有限公司

【摘要】 采用响应面法优化酶法山药粘液质多糖的脱蛋白工艺条件,确定最佳条件为:pH 8.0,时间2.0 h,液料体积质量比80 m L/g,酶底质量比30%,温度37℃;此条件下的蛋白脱除率为83.4%,多糖保留率为92.7%。通过测定山药粘液质多糖对自由基的清除率,发现它对DPPH自由基和过氧化氢的清除率均可达到80%以上,对OH自由基的清除能力较弱;通过测定山药粘液质多糖在相对湿度43%和81%时的吸湿率、保湿率,表明其综合保湿性能略优于透明质酸钠。

【Abstract】 The response surface methodology was used to optimize the deproteinization technology of yam mucilage polysaccharide. The results indicated that the optimal conditions were pH 8.0,time2 h,liquid-solid ratio 80 mL/g,enzyme-substrate ratio 30% and temperature 37 ℃. Under these conditions,the protein removal rate and polysaccharide retention rate were 83.4% and 92.7%,respectively. Through testing the free-radical scavenging rates of yam mucilage polysaccharide,the results showed that yam mucilage polysaccharide’s abilities of scavenging DPPH free radicals and hydrogen peroxide were very strong,and the clearing rates could achieve 80% above;but its ability of scavenging OH free radical was weak. In 43% and 81% relative humidity,by testing the moisture absorption rate and moisture rate,the results showed that the comprehensive moisturizing property of yam mucilage polysaccharide was slightly better than that of sodium hyaluronate.

  • 【文献出处】 食品与生物技术学报 ,Journal of Food Science and Biotechnology , 编辑部邮箱 ,2019年09期
  • 【分类号】TQ281
  • 【被引频次】9
  • 【下载频次】552
节点文献中: 

本文链接的文献网络图示:

本文的引文网络