【摘要】 探究长期摄入酪氨酸氧化产物对大鼠空间学习记忆及其对海马组织的影响。采用4周龄清洁级SD大鼠40只,随机分为Control组(正常日粮)、Tyr组(添加0.44%酪氨酸)、TOP1组(添加0.22%酪氨酸氧化产物)、TOP2组(添加0.44%酪氨酸氧化产物)。饲喂24周后,用Morris水迷宫检测大鼠空间学习与记忆能力,测定海马组织中蛋白质和脂质氧化产物的含量,用RTPCR检测海马组织抗氧化和炎症相关基因的表达。结果显示,饲喂24周后,与Control组和Tyr组相比,TOP1组和TOP2组大鼠出现空间学习与记忆障碍;海马组织中MDA、4-HNE、Dityr、3-NT、Aβ40含量都显著升高(p<0.05);Nrf2、NF-κB和i NOS的mRNA表达也显著上调(p<0.05)。因此,酪氨酸氧化产物导致大鼠海马组织氧化损伤,蛋白质和脂质氧化产物积累,造成大鼠空间学习记忆能力受损。更多还原

【Abstract】 To explore the effects of tyrosine oxidation products(TOP) on the spatial learning and memory of rats and hippocampus. 40 SD rats are divided into four groups:Control(normal diet),Tyr group(add 0.44%Tyr),TOP1 group(add 0.22% tyrosine oxidation products) and TOP2 group(add0.44% tyrosine oxidation products). After 24-week feeding,Morris water maze is used to test the spatial learning and memory of rats. Protein and lipid oxidation products in hippocampus are measured. Expressions of antioxidation-and inflammation-related genes are detected using RT-PCR. The results show that,after 24-week feeding,compared with Control and Tyr group mice,TOP1 and TOP2 group rats showed impaired spatial learning and memory in Morris water maze;hippocampal MDA,4-HNE,Dityr,3-NT and Aβ40 are significantly increased(p<0.05);expressions of Nrf2、NF-κB and iNOS were significantly up-regulated(p<0.05). Thus,tyrosine oxidation products induce oxidative damage to rats hippocampus,accumulation of lipid and protein oxidation products in hippocampus,and impair the spatial learning and memory of rats.更多还原