【摘要】 目的探寻分子生物学因素及大气颗粒物在COPD患者病情发生发展过程中的作用。方法用通过生物信息学方法筛选出COPD患者和健康人外周血中LncRNA meg3,并检测表达水平,提高空气中细颗粒物的浓度后观察健康人和COPD患者住院时间是否有所改变。结果发现实验组患者体内外周血中单核细胞的meg3表达水平显著高于对照组(P<0.05);实验组和对照组患者在高浓度细颗粒物空气中暴露后,其住院时间均和空气中细颗粒物浓度呈正相关,且和提高前的住院时间有显著差异(P<0.05)。结论 COPD的发生、发展和LncRNA meg3水平及大气中细颗粒的水平多少呈正相关。更多还原

【Abstract】 Objective To explore effect of molecular biological factors and atmospheric fine particles in development of COPD patients. Methods Choose LncRNA meg3 in peripheral blood of COPD patients and healthy people wiht bioinformatics method, and detect expression level. After increasing concentration of atmospheric fine particles, observe hospital stay changes of healthy people and COPD patients. Results Showed meg3 expression level in peripheral blood mononuclear cells in experimental group was significantly higher than control group(P< 0.05). In high concentration atmosphere with fine particles, hospitalization time of experimental and control group was positively correlated with concentration of atmospheric fine particles, and there was significant difference from that before increase,(P< 0.05). Conclusion Occurrence and development of COPD is positively correlated with level of LncRNA meg3 and atmospheric fine particles.更多还原