【摘要】 目的通过研究不同成分菌群移植后腹泻型SD大鼠模型的症状改善率(体重增长量、腹泻抑制率)、炎症指标及腹泻缓解天数,进一步探讨标准化菌群移植治疗对腹泻型模型的有效性与安全性。方法选取7周左右大小的SD雄性大鼠作为研究对象,随机分为空白对照组、模型对照组、成分菌群组、标准化菌群组,每组各10只;除空白对照组外,其余3组使用番泻叶复制大鼠腹泻模型。标准化粪菌供体采用空白对照组采集的新鲜粪便;成分菌群由4种常见的益生菌混合而成;模型对照组由生理盐水代替。腹泻使用灌胃方法每2天1次,一共3次;治疗前后一周内需检测并记录大鼠的体重、腹泻抑制率、炎症指标和腹泻缓解天数、肠道菌群丰度等,从以上几个方面对模型进行评价。结果菌群移植后,成分菌群组和标准化菌群组大鼠腹泻抑制率均明显升高,其中标准化菌群组腹泻抑制率升高最明显达67.30%。成分菌群组和标准化菌群组的炎症指标略低于模型对照组,差异具有显著性(Sidak’s multiple comparisons test:t=4.15, P<0.001,t=5.647, P<0.001)。成分菌群组和标准化菌群组的腹泻缓解天数均低于模型对照组,差异具有显著性(Dunnett’s multiple comparisons test:q=6.175, q=11.7, P<0.001)。与空白对照组大鼠体重增长量相比,其余各组体重量增长均降低,其中模型对照组最低为1.82%。各组肠道菌群分析结果微生物种类及含量无明显差异,其中标准化菌群组与正常大鼠肠道菌群类似。结论成分菌群和标准化菌群对大鼠腹泻模型均具有良好的治疗效果,其中成分菌群的治疗效果次于标准化菌群,为实际临床工作提出了可能的理论指导。在有条件使用标准化菌群移植的时候,排除禁忌后优选选择标准化菌群移植。本实验属于小样本动物模型实验,后期需要大样本动物及临床实验来验证,为临床试验提供了一个很好的指导思路,具有一定的参考价值。更多还原

【Abstract】 Objective By studying the diarrhea symptom improvement rate(weight gain, diarrhea inhibition rate), inflammation index and diarrhea alleviation days of diarrhea-type SD rat model after transplantation of different components, the effectiveness and the safetiness of standardized bacterial transplantation therapy on diarrhea model was further explored. Methods SD male rats of about 7 weeks old were selected as the research subjects, and were randomly divided into blank control group, model control group,component bacteria group and standardized bacteria group, each group consisting of 10; except the blank control group, the other three groups Rat diarrhea model was replicated using senna. The standardized fecal donors were fresh feces collected from the blank control group; the constituent flora was composed of fore common probiotics; the model control group was replaced by physiological saline.The rats were treated once every 2 days for a total of 3 times. The model was evaluated by measuring and recording the body weight,diarrhea inhibition rate, inflammation index and diarrhea alleviation days in the week before and after treatment. Results After the bacterial transplantation, the inhibition rate of diarrhea in the group of the bacteria group and the standardized group was significantly increased, and the inhibition rate of the diarrhea in the standardized group was 67.30%. The inflammatory index of the component group and the standardized group was slightly lower than that of the model control group, and the difference was significant(Sidak’s multiple comparisons test: t=4.15, t=5.647, P<0.001). The diarrhea alleviation days of the component group and the standardized group were lower than the model control group, and the difference was significant(Dunnett’s multiple comparisons test: q=6.175, q=11.7,P<0.001). Compared with the weight gain of the blank control group, the weight gain of the other groups decreased, and the lowest in the model control group was 1.82%. There was no significant difference in the microbial species and content of the intestinal flora analysis in each group, and the standardized bacterial group was similar to the normal intestinal flora.Conclusion The component flora and the standardized flora have a good therapeutic effect on the rat diarrhea model. The therapeutic effect of the component flora is inferior to the standardized flora, which provides a theoretical guidance for the actual clinical work. When conditional colonization is used conditionally, standardized colony transplantation is preferred after exclusion of contraindications. This experiment belongs to the small sample animal model experiment. It needs to be verified by large sample animals and clinical experiments in the later stage, which provides a good guiding idea for clinical trials and has certain reference value.更多还原