【摘要】 目的探讨壳寡糖对白血病K562细胞生长抑制和凋亡诱导作用。方法用不同浓度壳寡糖作用于K562细胞,CCK-8法观察壳寡糖对K562细胞生长增殖的影响,流式细胞仪检测壳寡糖对K562细胞凋亡的影响。结果壳寡糖能有效抑制K562细胞的增殖,呈时间-剂量依赖性;Annexin VIPI双标记流式细胞术检测结果显示,壳寡糖处理K562细胞能明显诱导凋亡,并呈剂量依赖性。结论壳寡糖可抑制K562细胞增殖并诱导其凋亡。更多还原

【Abstract】 Objective To Study chitosan oligosaccharides of growth inhibition effect and inducing apoptosis on leucocythemia K562 cells. Methods The effect of different concentration of chitosan oligosaccharides on the growth and colonization of K562 cells was observed by CCK-8 method. The effect of chitosan oligosaccharides on apoptosis of K562 cells was detected by fiow cytometry.Results Chitosan oligosaccharides could inhibit the proliferation of K562 cells in a time and dose-dependent manner.Annexin VIPIdouble-labeled flow cytometry showed K562 cells treated with chitosan oligosaccharides were able to induce apoptosis in dosedependent manner. Conclusion Chitosan oligosaccharides can inhibit the proliferation and induce apoptosis of K562 cells.更多还原