【摘要】 黄海是西太平重要的边缘海。微生物群落在有机污染物生物地球化学循环中起着十分关键的作用,它们的群落结构、多样性及变化一定程度上反映了沉积物质量的变化。宏基因组学能够在整体水平解析微生物群落结构,真实地揭示原位环境中微生物群落的复杂性和多样性。2016年10月对黄海西部海域19个监测站位的微生物群落及环境因子开展了深入调查。应用Illumina MiSeq宏基因组高通量测序技术,共鉴定海域沉积物中微生物41门266属,变形菌门为明显的优势门类,其相对丰度占总数的47%。其他较为丰富的门类包括酸杆菌门(Acidobacteria),放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes),浮霉菌门(Planctomycetes)和沈微菌门(Verrucomicrobia)。各站位细菌多样性非常高,但站位间差异较大。其中Chaol指数均值为7 365.3;Shannon指数平均为9.65;Simpson指数平均为0.995。应用Past软件,采用CCA分析法研究细菌群落与环境因子的相关性,结果显示总磷和沉积物粒径对微生物门水平的群落分布具有显著性影响。计算细菌与环境因子间Pearson相关系数并绘制热图分析,发现酸杆菌、衣原体(Chlamydiae)和硝化螺旋菌(Nitrospirae)与总磷呈较明显的负相关。更多还原

【Abstract】 The Yellow Sea is an important marginal sea of the western Pacific Ocean.Microbial communities play a key role in the biogeochemical cycling of organic pollutants.Their community structure and diversity,and the changes may reflect certain aspects of the sedimental quality.Metagenomic analysis can reveal the integral community structure,and unveil the in situ complexity and diversity of environmental microbial communities.In this study,an in-depth investigation was conducted on the microbial community and environmental factors of 19 monitoring stations in the western Yellow Sea in October,2016.Based on the Illumina Miseq high throughput sequencing technology,a total of 266 genera,comprising 41 phyla,of Archaea and Bacteria were detected from the sediment samples.Proteobacteria was the dominant group,comprising 47% of the total abundance.Other abundant groups included the phylum Acidobacteria,Actinobacteria,Bacteroidetes,Planctomycetes and Verrucomicrobia.The overall bacterial diversity was high,with large variation between stations.The average value of the Chaol index was 7 365.3;the Shannon index was9.65 on average,and the Simpson index was 0.995.CCA analysis using Past software showed significant correlation between the total phosphorus and sediment particle size,and the bacterial community,indicating their potential impacts on the microbial community.Pearson’s correlation coefficient analysis further revealed significant negative correlations between total phosphorus and several bacterial phyla,including Acidobacteria,Chlamydiae and Nitrospirae.更多还原