【摘要】 随着现代生态学的内涵延伸,植物个体功能性状在种群、群落和生态系统尺度上,都已成为明确植物个体和生态系统与环境协同变化规律的可靠途径。通过透射电镜法观察了黄瓜叶片的组织形态,并测定了黄瓜叶片化学组成和氮代谢相关酶活性。结果表明:黄瓜叶片的组织形态与化学组成之间具有协变关系。无氮和高氮胁迫条件下,黄瓜叶片中N, P, K含量较低,相对应的是叶绿体中片层结构混乱,脂质小球数量多,并且高氮条件下,叶肉细胞中淀粉粒数量显著增加(P <0.05)。相关性分析结果表明,黄瓜叶片生理性状、养分组成与土壤理化性质之间均存在关联。黄瓜叶片酶活性与土壤碱解氮和pH相关性较强。CAT, NR和GS活性均与土壤碱解氮含量显著正相关,MDA活性与土壤碱解氮含量显著负相关(P <0.05),CAT和GS活性与土壤pH值显著负相关。研究以个体生态学特征为依据,明确了日光温室黄瓜叶片功能性状对不同施肥策略的响应规律,弥补了传统群体生态学应用方面的不足,对于提升日光温室农业生态服务功能具有指导意义。更多还原

【Abstract】 The histological morphology of cucumber leaves was observed with the transmission electron microscopy,and the chemical composition and enzyme activities related with nitrogen(N) metabolism were also measured. The results showed that with the change of N input level, there was a covariant relationship between the histological morphology and chemical composition of cucumber leaves. Under the stress of N at zero and high N levels, the contents of N, phosphorus(P) and potassium(K) in cucumber leaves became relatively low, corresponding to the chaotic lamellar structure and vastly more plastoglobulus in chloroplasts. Meanwhile, the number of starch grain in mesophyll cells increased significantly(P < 0.05). The activities of CAT, NR and GS were significantly positively(P <0.05) correlated with the content of soil alkali-hydrolysis N and the MDA activities were significantly negatively(P <0.05) correlated with soil alkali-hydrolysis N content, while the activities of CAT and GS were significantly negatively(P < 0.05) correlated with soil pH value. Based on the characteristics of the individual ecology, the results clarified the response rule of the functional characteristics of greenhouse cucumber leaves to different fertilization strategies, made up the shortage of traditional population ecology application, and played a guide role in improving the agricultural ecological service function of solar greenhouse.更多还原