【摘要】 目的研究β-五没食子酰葡萄糖(β-PGG)对人胰腺癌细胞糖酵解途径的影响及细胞生长的调节潜能。方法用不同浓度的β-PGG(0、5、25、50、100μmol/L)处理人胰腺癌MiaPaCa-2细胞,采用CCK-8法检测β-PGG对人胰腺癌MiaPaCa-2细胞的增殖作用,细胞化学试剂盒检测糖酵解的产物乳酸的生成,蛋白印迹法检测糖酵解的上游调节子缺氧诱导因子-1α(HIF-1α)和葡萄糖转运蛋白1(GLUT1)以及糖酵解酶己糖激酶-Ⅱ(HK-Ⅱ)和磷酸果糖激酶(PFK)的蛋白表达水平。结果 CCK-8结果显示,β-PGG可以抑制人胰腺癌MiaPaCa-2细胞的增殖,其抑制作用呈现出剂量和时间依赖性(P<0.05)。检测细胞上清液的乳酸发现,无论是在常氧或缺氧条件下,β-PGG均能抑制乳酸的生成(P<0.05)。与对照组相比,β-PGG能抑制HIF-1α、GLUT1、HK-Ⅱ和PFK的表达水平,且抑制作用随着β-PGG浓度的增高而增加(P<0.05)。结论β-PGG可以抑制人胰腺癌细胞系MiaPaCa-2的糖酵解与增殖,其抑制作用可能与抑制MiaPaCa-2细胞中HIF-1α、GLUT1、HK-Ⅱ和PFK的蛋白表达有关。更多还原

【Abstract】 Objective To study the effect of penta-O-galloyl-β-D-glucose(β-PGG) on the glycolysis and growth of human pancreatic cancer cells.MethodsMiaPaCa-2 human pancreatic cancer cells were treated with different concentrations of β-PGG. The proliferation of MiaPaCa-2 was assessed by CCK8 method. Cytochemical kit was used to determine the product of glycolysis,lactate. Hypoxia inducible factor-1α(HIF-1α),glucose transporter 1(GLUT1),the glycolytic enzymes of hexokinase Ⅱ(HK-Ⅱ) and phosphofructokinase(PFK) were determined by Western blot assay.ResultsThe results of CCK8 assay showed that β-PGG inhibited the proliferation of pancreatic cancer MiaPaCa-2 cells in a dose-and time-dependent manner(P<0.05). β-PGG also inhibited the production of lactate in both normoxic andhypoxia conditions(P<0.05). Compared with the control group,β-PGG inhibited the expression of HIF-1α,GLUT1,HK-Ⅱ and PFK. And the inhibitory effect increased with the increased concentration of β-PGG(P<0.05).Conclusionβ-PGG can inhibit the glycolysis pathway of human pancreatic cancer cell line MiaPaCa-2 and also inhibit the proliferation ofMiaPaCa-2 cells. The inhibition may be achieved by inhibiting the expressions of HIF-1α,GLUT1,HK-Ⅱ and PFK incancer cells.更多还原