【摘要】 目的:探讨白介素(IL)-17和IL-35在系统性红斑狼疮(SLE)中的作用及其与肾损害的关系。方法:采用酶联免疫吸附试验(ELISA)检测55例SLE患者和25名健康者血清IL-17和IL-35水平,比较SLE患者与对照者、SLE活动组与SLE非活动组、狼疮肾炎(LN)患者与非LN患者中两种细胞因子的差异,分析SLE患者血清IL-17、IL-35、SLE疾病活动指数(SLEDAI)评分的相关性以及LN患者血清IL-17、IL-35与尿蛋白的相关性。结果:与对照者相比,SLE患者血清IL-17水平升高而IL-35水平降低(P<0.01),且SLE活动组的血清IL-17水平高于非活动组(P<0.01),IL-35水平低于非活动组(P<0.01)。SLE患者血清IL-17水平与SLEDAI评分正相关而IL-35水平与SLEDAI评分负相关,且血清IL-17水平与IL-35水平呈负相关。与非LN患者比较,LN患者的血清IL-17水平升高而IL-35水平减低(P<0.05),且LN患者尿蛋白定量(24 h)与血清IL-17水平正相关,与IL-35水平负相关。结论:IL-17和IL-35是一对紧密相关的促炎/抗炎细胞因子,血清IL-17水平升高、IL-35水平下降,两者的平衡失调,参与了SLE的疾病发生及其肾脏损伤过程。更多还原

【Abstract】 Objective: To investigate the serum IL-17 and IL-35 levels in patients with systemic lupus erythematosus(SLE), explore the effect of IL-17 and IL-35 on the pathogenesis of SLE and their relationship with renal damage. Methods: Serum IL-17 and IL-35 levels were quantified using ELISA in 55 patients with SLE and 25 healthy controls, then the levels were compared between SLE patients and healthy controls, active SLE patients and inactive SLE patients, and SLE patients with nephritis and without nephritis, the correlations among serum IL-17, IL-35 and SLEDAI scores in SLE patients were analyzed, and the relationship of serum IL-17 and IL-35 levels with24-h proteinuria were also analyzed in lupus nephritis(LN) patients. Results: Serum IL-17 levels were significantly higher and IL-35 levels were significantly lower(P<0.01) in patients with SLE compared with healthy controls.And compared with inactive SLE patients, active SLE patients had higher serum IL-17 levels(P<0.01) and serum IL-35 levels were decreased in active SLE patients(P<0.01). A positive correlation was observed between serum IL-17 levels and SLEDAI scores and IL-35 levels were negatively correlated with SLEDAI scores in SLE patients. Furthermore, the expression of IL-17 and IL-35 were negatively correlated with each other. Compared with SLE patients without nephritis, serum IL-17 levels were significantly higher and IL-35 levels were decreased in lupus nephritis(LN) patients(P<0.05).Moreover, 24-h proteinuria was positively correlated with serum IL-17 levels and negatively with IL-35 levels in LN patients. Conclusion:IL-17 has strong proinflammatory effects and IL-35 is identified as anti-inflammatory cytokines and they are strongly associated with each other, serum IL-17 levels increase and IL-35 levels decrease in SLE patients, and the imbalance between IL-17 and IL-35 may be involved in pathogenesis of SLE and renal damage.更多还原