【摘要】 目的:观察富含丝氨酸/精氨酸剪接因子2(SRSF2)、HMGA2和Caspase-3在卵巢高级别浆液性癌(HGSOC)及其前驱病变中的表达并探讨其意义。方法:收集输卵管分泌型上皮细胞过度生长(SCOUT)20例、P53印记13例、浆液性输卵管上皮内癌(STIC)18例、输卵管高级别浆液性癌37例和HGSOC 48例,应用免疫组化技术检测SRSF2、HMGA2和Caspase-3在5组中的表达,分析3个指标间及其与临床病理参数间的关系。结果:在5组中SRSF2和Caspase-3呈递减性表达,HMGA2呈递增性表达(P均<0.05)。3个指标在STIC、输卵管高级别浆液性癌和HGSOC中的表达无显著差异(P均>0.05)。在HGSOC及其前驱病变中,SRSF2与Caspase-3的表达呈正相关,SRSF2与HMGA2、Caspase-3与HMGA2的表达均呈负相关(r分别为0.618、-0.329和-0.373,P均<0.05)。3个指标的表达与肿瘤FIGO分期、肿瘤大小及年龄无关。结论:SRSF2和HMGA2蛋白异常表达可能协同参与调控HGSOC肿瘤细胞凋亡。更多还原

【Abstract】 Objective: To examine the expression of SRSF2, HMGA2 and Caspase-3 in high grade serous ovarian carcinoma and precursor lesions,and to discuss their relevant meanings. Methods: The expressions of SRSF2, HMGA2 and Caspase-3 in the tissue inclding high grade serous ovarian carcinoma(HGSOC,48 cases), fallopian tube high grade serous fallopian tube carcinoma(37 cases), serous tubal intraepithelial carcinoma-STIC(18 cases), P53 signature(13 cases)and secretory cell outgrowths(SCOUT,20 cases)were detected using immunohistochemical method. Relations between the three markers as well as relations beween markers and clinical pathological parameters were analyzed. Results:(1)The expressions of SRSF2 and Caspase-3 in the SCOUT, P53 signature, STIC, ovarian/fallopian tube high grade serous carcinoma showed a decreasing trend, while the expressions of HMGA2 showed an increasing trend. The differences were statistically significant(P<0.05). There were no significant differences in expressions of the three markers between STIC, fallopian tube high grade serous carcinoma and HGSOC.(2)In HGSOC and its precursor lesion, positive correlation between the expression of SRSF2 and Caspase-3,and negative correlations beween SRSF2 and HMGA2, Caspase-3 and HMGA2 were obtained(They values 0.618、-0.329 and-0.373 respectively,P<0.05);There was no correlation between the expressions of the three markers and FIGO stage, tumor size and age.Conclusion: The abnormal expressions of SRSF2 and HMGA2 may be involed in regulating apoptosis pathway synergisticly in HGSOC.更多还原