【摘要】 目的:探讨miR-199a-3p在胰腺癌中的表达水平及作为胰腺癌的诊断指标的意义,并进行生物信息学分析。方法:从GEO数据库收集胰腺癌和正常胰腺组织中miR-199a-3p的数据。汇总数据绘制受试者工作特征曲线,进行Meta分析,采用生物信息学方法预测miR-199a-3p的靶基因,并对靶基因进行GO功能富集分析和KEGG通路富集分析。结果:收集到6个GEO数据,4个数据的ROC曲线表现出显著的诊断意义,Meta分析结果显示miR-199a-3p在胰腺癌中表达水平高于正常胰腺组织,差异有统计学意义(P<0.001)。预测到221个miR-199a-3p潜在的靶基因,其功能主要富集在基因表达的调控,细胞代谢的调控,蛋白质域特异性结合等生物学过程(P<0.001),KEGG分析结果显示主要集中在PI3K-Akt通路、黏着斑、肌动蛋白细胞骨架调控、癌症通路、Hippo通路、鞘脂通路、ErbB通路、MAPK通路等通路(P<0.05)。结论:相比正常胰腺组织,miR-199a-3p在胰腺癌为高表达,可作为胰腺癌的一个潜在诊断指标,并可能在胰腺癌的发生发展中起到重要作用,为胰腺癌的研究和治疗提供了新的方向。更多还原

【Abstract】 O bjective: To investigate miR-199 a-3 p’s expression levels and diagnostic value in pancreatic cancer, and to conduct bioinformatics analysis. Methods:We gathered miR-199 a-3 p expression data of pancreatic cancer and normal pancreatic tissues by analysis of a public dataset from Gene Expression Omnibus(GEO) databases. We plotted ROC curve and conducted Meta-analysis using all collected data. Furthermore, the bioinformatics analysis of miR-199 a-3 p involved target prediction analysis, gene ontology enrichment and KEGG pathway enrichment. Results: A total of six GEO data were finally collected in this study, four of these data showed significant diagnostic value by conducting ROC curve,and Meta-analysis indicated that miR-199 a-3 p expression was significantly higher in pancreatic cancer by GEO data compared with normal pancreatic tissue(P<0.001). The 221 target genes were predicted as potential target genes for miR-199 a-3 p. The function of target genes was mostly enriched in the regulation of cell metabolism, regulation of gene expression, protein domain specific binding and other biological processes(P<0.001). KEGG analysis was mostly observed in enrichment pathway, such as PI3 K-Akt signaling pathway, focal adhesion, actin cytoskeleton regulation, cancer pathway, Hippo signaling pathway,sphingolipid signaling pathways, ErbB signaling pathway, MAPK signaling pathway and other Signal pathways(P<0.05). Conclusion: Mi R-199 a-3 p expression is significantly higher in pancreatic cancer compared with normal pancreatic tissues. It can be used as a potential diagnostic biomarker of pancreatic cancer, and it may play a vital role in the development of pancreatic cancer. Meanwhile, it could provide a research direction for the treatment of pancreatic cancer in the future.更多还原