【摘要】 雨生红球藻是一种重要的资源微藻,产物虾青素具有超强抗氧化活性,因而广泛被应用于医药和食品等领域。为雨生红球藻实现高产虾青素,研究了3种胁迫条件,光照强度(5 000、7 000、9 000 lx)、硝酸钠浓度(0、0.13、0.25 g/L)和氯化钠浓度(0、2.5、5.0 g/L)对雨生红球藻的虾青素含量的影响,利用响应面法对胁迫条件进行优化。3因素对产生虾青素含量的影响顺序为:氯化钠浓度>光照强度>硝酸钠浓度。优化得到的最佳提取条件为光照强度9 000 lx、硝酸钠浓度0 g/L、氯化钠浓度2.5 g/L,在此条件下虾青素含量可达6.8 mg/L,与预测值相近,表明模型具有较高可靠性。更多还原

【Abstract】 Haematococcus pluvialis is an important resource microalgae. The product astaxanthin has excellent antioxidant activity and is widely used in medicine and food. In order to study the high-yield astaxanthin of Haematococcus pluvialis, three stress conditions(5 000, 7 000, 9 000 lx), sodium nitrate concentrations(0, 0.13, 0.25 g/L)and chlorination were studied. The effects of sodium concentrations(0, 2.5, 5.0 g/L)on astaxanthin content of Haematococcus pluvialis were optimized by response surface methodology. The order of influence of the three factors on the production of astaxanthin content was: sodium chloride concentration>light intensity>sodium nitrate concentration. The optimal extraction conditions were: light intensity 9 000 lx, sodium nitrate concentration 0 g/L, sodium chloride concentration 2.5 g/L, under which the astaxanthin content could reach 6.8 mg/L, and it was similar to the predicted value. The results indicated that the model has high reliability.更多还原