【摘要】 WRKY转录因子家族在植物抗逆反应中发挥着重要的作用。本研究以抗白粉病小麦Brock为材料,从中克隆到一个WRKY基因,该基因全长1 485 bp,预测ORF长1 392 bp,编码463个氨基酸,分子量为49.8 kDa。经NCBI/Blastx比对发现,该基因与粗山羊草中WRKY26基因（序列号XP₀20151406）所编码的转录因子同源性高达98%,故将其命名为TaWRKY26,并将扩增得到的序列全长上传至GenBank进行登记,序列号为MK358190。利用实时荧光定量PCR技术,对白粉菌胁迫后TaWRKY26在不同抗性小麦品种（抗病小麦Brock,感病小麦京411,抗病近等基因系Brock/京4117）中不同时间（0,2,4,8,12,24,48,72,120,168 h）的表达量进行分析,结果发现,在受到白粉菌侵染后,3个品种小麦的TaWRKY26表达量除12和72 hpi外,各时间段均表现为抗病小麦Brock>近等基因系Brock/京4117>感病小麦京411,抗白粉病小麦近等基因系Brock/京4117（抗病）中TaWRKY26表达趋势更偏向抗病亲本Brock。综合推测TaWRKY26在小麦对白粉菌侵染的应答反应过程中发挥了积极作用。更多还原

【Abstract】 The WRKY family plays an important role in the stress tolerance mechanism of plants. In this experiment, a gene of WRKY was cloned from the powdery mildew disease resistant wheat Brock. The gene contained a 1 392 bp ORF, and 463 amino acids with a predicted molecular weight of 49.8 k Da. The NCBI/Blastx alignment showed that this gene had 98% homology to the transcription factor encoded by the WRKY26 gene（SEQ ID: XP₀20151406） in Aegilops tauschii, so we named it TaWRKY26. The full length of the amplified sequence was uploaded to GenBank for registration, and the accession number was MK358190. The expression patterns of TaWRKY26 in different resistant wheat[disease resistant wheat Brock, susceptible wheat variety Jing411, and Near-isogenic Lines Brock/Jing 4117（NILs）] after infected by powdery mildew were studied through real-time fluorescent quantitative PCR, the expression quantity at different time（0, 2, 4, 8, 12, 24, 48, 72, 120, 168 h） was analyzed. After infected by powdery mildew, the expression quantity of TaWRKY26 at 0, 2, 4, 8, 24, 48, 120, 168 h were all shown as follows: Brock>NILs >Jing411,and the expressive trend in NILs was more closer to disease resistant wheat Brock, indicating that TaWRKY26 participated in the interaction of wheat and powdery mildew.更多还原