【摘要】 以磁性Fe₃O₄为内核,对磁性纳米粒子表面进行包裹和修饰,制备了对吡咯并喹啉醌-多巴胺（PQQ-DA）具有高识别性、高选择性的磁性纳米分子印迹材料,并结合超高效液相色谱-质谱（UPLC-MS）测定了生物体内PQQ-DA的含量。结果显示,PQQ-DA在0.02～0.1 mg/mL范围内呈良好的线性关系,相关系数（r²）为0.996 6,检出限为0.2×10^-11 mg/mL,加标回收率为88.7%～95.5%,相对标准偏差为2.6%～3.2%。该方法灵敏度高、重复性好,可用于生物体内PQQ-DA痕量产物的检测。更多还原

【Abstract】 A pyrroloquinoquinone（PQQ）-dopamine（DA）magnetic nanoparticle imprinted polymers were prepared by the combination of PQQ-DA and magnetic nano-Fe₃O₄ as the core wrapped with SiO₂,which possess high recognition and high selectivity toward PQQ-DA and have great advantages in the separation,purification and enrichment of low concentration compounds in complex matrix of brain tissue after molecular imprinting modification.The magnetic nanoparticles were applied in the determination of trace PQQ-DA in brain tissue samples by ultrahigh performance liquid chromatography-tandem mass spectrometry（UPLC-MS）.Results showed that,under the optimized conditions,there was a good linear relationship for PQQ-DA in the range of 0.02-0.1 mg/mL with a correlation coefficient（r²）of 0.996 6,and the detection limit was 0.2×10^-11 mg/mL.The recoveries for brain tissue samples were in the range of 88.7%-95.5%,with relative standard deviations（n=6）of 2.6%-3.2%.The method could be used in the detection of trace PQQ-DA in vivo with high sensitivity and good repeatability.更多还原