【摘要】 目的观察鹿茸多肽对阿霉素所致心肌损伤模型大鼠心肌特异性转录因子Nkx 2.5、GATA4、ATF-2、MEF-2C表达的影响。方法 Wistar大鼠60只随机分为6组,每组10只。腹腔注射阿霉素建立心肌损伤大鼠模型,连续给药21d。末次给药结束后1h,PowerLab数据采集分析系统检测各组大鼠心率及心电图ST段变化,Elisa法检测各组大鼠血清中Nkx2.5、GATA4、ATF-2和MEF-2C含量,Western-blot检测各组大鼠心脏中Nkx2.5、GATA4、ATF-2和MEF-2C蛋白表达水平。结果鹿茸多肽可明显降低阿霉素所致心肌损伤大鼠模型的心率,ST段上调幅度明显降低,升高大鼠血清中Nkx 2.5、GATA4和MEF-2C含量及上调心脏中蛋白水平(P<0.05或P<0.01),ATF-2无明显变化(P>0.05)。结论鹿茸多肽可明显缓解阿霉素所致的大鼠心肌损伤,其作用机制可能与调节心肌特异性转录因子Nkx 2.5、GATA4、MEF-2C水平有关。更多还原

【Abstract】 Objective To observe the effects of Velvet antler polypeptide on the expression of myocardial specific transcription factors Nkx 2.5, GATA4, ATF-2 and MEF-2 C in rats with myocardial injury induced by adramycin. Methods Sixty Wistar rats were randomly divided into 6 groups, 10 rats in each group. The rat model of myocardial injury was established by intraperitoneal injection of adramycin for 21 days. One hour after the last dose, PowerLab data acquisition and analysis system was used to detect the heart rate and ST segment changes of ECG in each group. Use Elisa to detect the contents of Nkx2.5, GATA4, ATF-2 and MEF-2 C in serum of each group. Use Western-blot to detect the levels of Nkx2.5, GATA4, ATF-2 and MEF-2 C proteins in the hearts of rats in each group were detected by blot.Results The Velvet antler polypeptide significantly reduced the heart rate of the rat model of myocardial injury induced by adramycin, and the ST-segment up-regulation was significantly decreased. The serum levels of Nkx 2.5, GATA4 and MEF-2 C were increased and the protein levels in the heart were up-regulated(P<0.05 or P<0.01), there was no significant change in ATF-2(P>0.05).Conclusion Velvet antler polypeptide can significantly alleviate myocardial injury induced by adramycin in rats, and its mechanism may be related to the regulation of myocardial specific transcription factors Nkx 2.5, GATA4 and MEF-2 C.更多还原